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Literature summary for 6.3.2.3 extracted from
- Enzymatic production of glutathione by bifunctional gamma-glutamylcysteine synthetase/glutathione synthetase coupled with in vitro acetate kinase-based ATP generation (2016), Appl. Biochem. Biotechnol., 180, 1446-1455 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gshF, recombinant expression in Escherichia coli strain Rosetta (DE3) coexpressing Escherichia coli acetate kinase (gene ack) | Streptococcus thermophilus |
| gene gshF, recombinant expression in Escherichia coli strain Rosetta (DE3), coexpression with acetate kinase (gene ack) from Lactobacillus sanfranciscensis | Streptococcus agalactiae serogroup V |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | enzymatic production of glutathione by recombinant cell-free bifunctional gamma-glutamylcysteine synthetase/glutathione synthetase (gamma-GCS-GS or GshF) coupled with in vitro acetate kinase-based ATP generation in Escherichia coli strain Rosetta (DE3), method optimization. The recombinant enzyme comprises both the activities of gamma-glutamylcysteine synthetase (gamma-GCS or GSHI, EC 6.3.2.2) and GSH synthetase (GS or GSHII, EC 6.3.2.3). The gshF from Streptomyces thermophilus shows poor expression levels compared to gshF from Streptomyces agalactiae. GSH production resulting from a combination of recombinant Escherichia coli BL21(DE3) expressing gshF from Streptomyces agalactiae with recombinant Escherichia coli BL21(DE3) expressing acetate kinase from Lactobacillus sanfranciscensis is 2.5 times higher than that of gshF from Streptomyces thermophilus | Streptococcus agalactiae serogroup V |
| additional information | enzymatic production of glutathione by recombinant cell-free bifunctional gamma-glutamylcysteine synthetase/glutathione synthetase (gamma-GCS-GS or GshF) coupled with in vitro acetate kinase-based ATP generation in Escherichia coli strain Rosetta (DE3), method optimization. The recombinant enzyme comprises both the activities of gamma-glutamylcysteine synthetase (gamma-GCS or GSHI, EC 6.3.2.2) and GSH synthetase (GS or GSHII, EC 6.3.2.3). The gshF from Streptomyces thermophilus shows poor expression levels compared to gshF from Streptomyces agalactiae. GSH production resulting from a combination of recombinant Escherichia coli BL21(DE3) expressing gshF from Streptomyces agalactiae with recombinant Escherichia coli BL21(DE3) expressing acetate kinase from Lactobacillus sanfranciscensis is 2.5 times higher than that of gshF from Streptomyces thermophilus with acetate kinase from Escherichia coli | Streptococcus thermophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Streptococcus thermophilus |  |
| Mg2+ | required | Streptococcus agalactiae serogroup V | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-L-glutamyl-L-cysteine + glycine | Streptococcus thermophilus | - |
ADP + phosphate + glutathione | - |
? | |
| ATP + gamma-L-glutamyl-L-cysteine + glycine | Streptococcus agalactiae serogroup V | - |
ADP + phosphate + glutathione | - |
? | |
| ATP + gamma-L-glutamyl-L-cysteine + glycine | Streptococcus agalactiae serogroup V ATCC BAA-611 / 2603 V/R | - |
ADP + phosphate + glutathione | - |
? | |
| ATP + gamma-L-glutamyl-L-cysteine + glycine | Streptococcus thermophilus SIIM B218 | - |
ADP + phosphate + glutathione | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus agalactiae serogroup V | Q8DXM9 | - |
- |
| Streptococcus agalactiae serogroup V ATCC BAA-611 / 2603 V/R | Q8DXM9 | - |
- |
| Streptococcus thermophilus | D4N891 | - |
- |
| Streptococcus thermophilus SIIM B218 | D4N891 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-L-glutamyl-L-cysteine + glycine | - |
Streptococcus thermophilus | ADP + phosphate + glutathione | - |
? | |
| ATP + gamma-L-glutamyl-L-cysteine + glycine | - |
Streptococcus agalactiae serogroup V | ADP + phosphate + glutathione | - |
? | |
| ATP + gamma-L-glutamyl-L-cysteine + glycine | - |
Streptococcus agalactiae serogroup V ATCC BAA-611 / 2603 V/R | ADP + phosphate + glutathione | - |
? | |
| ATP + gamma-L-glutamyl-L-cysteine + glycine | - |
Streptococcus thermophilus SIIM B218 | ADP + phosphate + glutathione | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GSH synthetase | - |
Streptococcus thermophilus |
| GSH synthetase | - |
Streptococcus agalactiae serogroup V |
| gshAB | - |
Streptococcus thermophilus |
| gshAB | - |
Streptococcus agalactiae serogroup V |
| GshF | - |
Streptococcus thermophilus |
| GshF | - |
Streptococcus agalactiae serogroup V |
| GSHII | - |
Streptococcus thermophilus |
| GSHII | - |
Streptococcus agalactiae serogroup V |
| More | cf. Ec 6.3.2.2 | Streptococcus thermophilus |
| More | cf. Ec 6.3.2.2 | Streptococcus agalactiae serogroup V |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptococcus thermophilus |
| 30 | - |
assay at | Streptococcus agalactiae serogroup V |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Streptococcus thermophilus |
| 6.8 | - |
assay at | Streptococcus agalactiae serogroup V |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Streptococcus thermophilus | |
| ATP | - |
Streptococcus agalactiae serogroup V | |
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Release 2023.1 (January 2023)
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