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Literature summary for 6.3.2.3 extracted from
- Site directed mutagenesis of Schizosaccharomyces pombe glutathione synthetase produces an enzyme with homoglutathione synthetase activity (2012), PLoS ONE, 7, e46580.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| sequence comparison, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain DH5alpha | Schizosaccharomyces pombe |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A485L/T486P | site-directed mutagenesis, the mutant shows 70% reduced activity compared to the wild-type activity, and a shift of substrate specificity with increased affinity of GSH2 for Ser as a substrate, while affinity to Gly is preserved. This provides another biosynthetic pathway for hydroxymethyl glutathione, which is known to be synthesized from glutathione and Ser in a reaction catalysed by carboxypeptidase Y | Schizosaccharomyces pombe |
| I471M/C472M/A485L/T486P | site-directed mutagenesis, the mutant shows 38% reduced activity compared to the wild-type activity, and a shift of substrate specificity with 1.2fold increased affinity of GSH2 for beta-Ala and lowered affinity for Gly, which is a characteristic of the enzyme homoglutathione synthetase found in plants, EC 6.3.2.23 | Schizosaccharomyces pombe |
| I471M/C472V | site-directed mutagenesis, the mutant shows showed much lower affinity towards Gly and 78% reduced activity compared to the wild-type activity, but no other differences | Schizosaccharomyces pombe |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Schizosaccharomyces pombe |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, about, recombinant His-tagged wild-type and mutant enzymes, SDS-PAGE | Schizosaccharomyces pombe |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-L-glutamyl-L-cysteine + glycine | Schizosaccharomyces pombe | - |
ADP + phosphate + glutathione | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces pombe | P35669 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain DH5alpha by nickel affinity chromatography, gel filtration, and Cibacron blue 3GA affinity chromatography | Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-L-glutamyl-L-cysteine + glycine | - |
Schizosaccharomyces pombe | ADP + phosphate + glutathione | - |
? | |
| additional information | no activity with beta-alanine and L-serine by wild-type glutathione synthetase | Schizosaccharomyces pombe | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, about, recombinant His-tagged wild-type and mutant enzymes, SDS-PAGE | Schizosaccharomyces pombe |
| More | structure comparisons and homology structure molecular modelling of the GSH2 wild-type and mutant enzymes, overview | Schizosaccharomyces pombe |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glutathione synthetase | - |
Schizosaccharomyces pombe |
| GSH2 | - |
Schizosaccharomyces pombe |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
assay at | Schizosaccharomyces pombe |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Schizosaccharomyces pombe | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | homoglutathione synthetase, EC 6.3.2.23, has evolved from glutathione synthetase by a single gene duplication event | Schizosaccharomyces pombe |
| metabolism | A485L/T486P mutant shows a shift the substrate specificity increased affinity of GSH2 for Ser as a substrate, while affinity to Gly is preserved. This provides a new biosynthetic pathway for hydroxymethyl glutathione, which is known to be synthesized from glutathione and Ser in a reaction catalysed by carboxypeptidase Y | Schizosaccharomyces pombe |
| additional information | structure comparisons and homology structure molecular modelling of the GSH2 wild-type and mutant enzymes, overview | Schizosaccharomyces pombe |
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