Protein Variants | Comment | Organism |
---|---|---|
V44A | decrease in melting temperature, slight decrease in activity | Homo sapiens |
V44A/V45A | decrease in melting temperature, initial activity similar to wild-type | Homo sapiens |
V44W | decrease in melting temperature, 16% decrease in activity | Homo sapiens |
V45A | decrease in melting temperature, slight decrease in activity | Homo sapiens |
V45W | decrease in melting temperature, 30% decrease in activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
gamma-L-Glu-aminobutanoate | mutant V45W, pH 8.2, 37°C | Homo sapiens | |
0.95 | - |
gamma-L-Glu-aminobutanoate | mutant V44W, pH 8.2, 37°C | Homo sapiens | |
1.42 | - |
gamma-L-Glu-aminobutanoate | mutant V44A/V45A, pH 8.2, 37°C | Homo sapiens | |
1.42 | - |
gamma-L-Glu-aminobutanoate | wild-type, pH 8.2, 37°C | Homo sapiens | |
1.54 | - |
gamma-L-Glu-aminobutanoate | mutant V45A, pH 8.2, 37°C | Homo sapiens | |
1.6 | - |
gamma-L-Glu-aminobutanoate | mutant V44A, pH 8.2, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-L-Glu-aminobutanoate + Gly | - |
Homo sapiens | ADP + phosphate + ophthalmic acid | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
41.3 | - |
melting temperature, mutant V45W | Homo sapiens |
42.8 | - |
melting temperature, mutant V44A/V45A | Homo sapiens |
47.8 | - |
melting temperature, mutant V45A | Homo sapiens |
51.1 | - |
melting temperature, mutant V44A | Homo sapiens |
57 | - |
melting temperature, mutant V44W | Homo sapiens |
60.4 | - |
melting temperature, wild-type | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.6 | - |
gamma-L-Glu-aminobutanoate | mutant V45W, pH 8.2, 37°C | Homo sapiens | |
16.4 | - |
gamma-L-Glu-aminobutanoate | mutant V44W, pH 8.2, 37°C | Homo sapiens | |
17.4 | - |
gamma-L-Glu-aminobutanoate | mutant V45A, pH 8.2, 37°C | Homo sapiens | |
18.9 | - |
gamma-L-Glu-aminobutanoate | mutant V44A, pH 8.2, 37°C | Homo sapiens | |
19.5 | - |
gamma-L-Glu-aminobutanoate | wild-type, pH 8.2, 37°C | Homo sapiens | |
21.7 | - |
gamma-L-Glu-aminobutanoate | mutant V44A/V45A, pH 8.2, 37°C | Homo sapiens |