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Literature summary for 6.3.2.3 extracted from
- Overexpression of glutathione synthetase in Indian mustard enhances cadmium accumulation and tolerance (1999), Plant Physiol., 119, 73-79.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | overexpression of the enzyme in transgenic plants offers a promising strategy for the production of plants with superior heavy-metal phytoremediation capacity | Brassica juncea |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Brassica juncea plants via infection with Agrobacterium tumefaciens, under control of the 35S cauliflower mosaic virus promotor | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of transgenic plants by overexpression of the enzyme in the cytosol of Brassica juncea, i.e. Indian mustard, transgenic plants show 3fold increased ability in the shoot compared to the wild-type to accumulate and tolerate heavy metals, e.g. cadmium, for use in detoxification | Escherichia coli |
| additional information | construction of transgenic plants by overexpression of the Escherichia coli enzyme, encoded by gene gshII, in the cytosol of transgenic plants, the plants show 3fold increased ability in the shoot compared to the wild-type to accumulate and tolerate heavy metals, e.g. cadmium | Brassica juncea |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | transgenic plants overexpressing the enzyme from Escherichia coli show enhanced tolerance against cadmium, 3fold in the shoot | Brassica juncea |  |
| additional information | transgenic plants overexpressing the enzyme from Escherichia coli show enhanced salt tolerance | Brassica juncea |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 38000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-Glu-L-Cys + Gly | Escherichia coli | - |
ADP + phosphate + glutathione | - |
ir | |
| ATP + gamma-Glu-L-Cys + Gly | Brassica juncea | - |
ADP + phosphate + glutathione | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica juncea | - |
indian mustard | - |
| Escherichia coli | - |
gene gshII | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Brassica juncea | - |
| seed | - |
Brassica juncea | - |
| shoot | - |
Brassica juncea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-Glu-L-Cys + Gly | - |
Escherichia coli | ADP + phosphate + glutathione | - |
ir | |
| ATP + gamma-Glu-L-Cys + Gly | - |
Brassica juncea | ADP + phosphate + glutathione | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glutathione synthetase | - |
Escherichia coli |
| Glutathione synthetase | - |
Brassica juncea |
| GS | - |
Escherichia coli |
| GS | - |
Brassica juncea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
| ATP | - |
Brassica juncea | |
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Release 2023.1 (January 2023)
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