Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Rattus norvegicus | |
0.037 | - |
ATP | recombinant enzyme, pH 8.2, 37°C | Rattus norvegicus | |
0.042 | - |
gamma-L-Glu-L-alpha-aminobutyrate | recombinant enzyme, pH 8.2, 37°C | Rattus norvegicus | |
0.913 | - |
glycine | recombinant enzyme, pH 8.2, 37°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-L-Glu-L-Cys + Gly | Rattus norvegicus | second step in the biosynthesis of glutahione | ADP + phosphate + glutathione | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli BL21(DE3), to homogeneity | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione | substrate binding kinetics and mechanism, negative cooperativity of gamma-L-Glu-L-Cys | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
purified recombinant enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-L-Glu-L-alpha-aminobutyrate + Gly | - |
Rattus norvegicus | ADP + phosphate + glutathione | - |
r | |
ATP + gamma-L-Glu-L-Cys + Gly | wild-type enzyme shows negative cooperativity in binding of gamma-L-Glu-L-Cys | Rattus norvegicus | ADP + phosphate + glutathione | - |
r | |
ATP + gamma-L-Glu-L-Cys + Gly | second step in the biosynthesis of glutahione | Rattus norvegicus | ADP + phosphate + glutathione | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | one active site for each subunit | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
Glutathione synthetase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
ATP | recombinant enzyme, pH 8.2, 37°C | Rattus norvegicus | |
13 | - |
glycine | recombinant enzyme, pH 8.2, 37°C | Rattus norvegicus | |
13 | - |
gamma-L-Glu-L-alpha-aminobutyrate | recombinant enzyme, pH 8.2, 37°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |