Crystallization (Comment) | Organism |
---|---|
mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
G240V | mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region | Escherichia coli |
P227V | mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region | Escherichia coli |
P227V/G240V | mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region | Escherichia coli |
General Stability | Organism |
---|---|
the remaining activity after arginyl-endopeptidase treatment is higher in the presence of ATP and/or gamma-Glu-Cys than in their absence | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values for Gly and gamma-Glu-Cys are sensitive to the mutation and drastically increased | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type and mutant enzymes | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-Glu-L-Cys + Gly | - |
Escherichia coli | ADP + phosphate + glutathione | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover number is fatally reduced in the P227V/G240V mutant | Escherichia coli |