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Literature summary for 6.3.2.3 extracted from

  • Gali, R.R.; Board, P.G.
    Identification of an essential cysteine residue in human glutathione synthase (1997), Biochem. J., 321, 207-210.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.3

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
C294A mutant enzymes Cys294Ala and Cys409Ala retain significant residual activity. Substantial decreases in activity are detected with mutant Cys522Ala and Cys-free mutant Cys294/Cys409/Cys422 to Ala294/Ala409/Ala422 Homo sapiens
C409A mutant enzymes Cys294Ala and Cys409Ala retain significant residual activity. Substantial decreases in activity are detected with mutant Cys522Ala and Cys-free mutant Cys294/Cys409/Cys422 to Ala294/Ala409/Ala422 Homo sapiens
C522A mutant enzymes Cys294Ala and Cys409Ala retain significant residual activity. Substantial decreases in activity are detected with mutant Cys522Ala and Cys-free mutant Cys294/Cys409/Cys422 to Ala294/Ala409/Ala422 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Homo sapiens
iodoacetate
-
 Homo sapiens
NEM
-
 Homo sapiens
PCMB
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + gamma-Glu-L-Cys + Gly Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 wild-type and mutant enzymes: Cys294Ala, Cys409Ala, Cys422Ala and Cys294/Cys409/Cys422 to Ala294/Ala409/Ala422
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gamma-Glu-L-Cys + Gly
-
 Homo sapiens ADP + phosphate + glutathione
-
 ?
ATP + gamma-Glu-L-Cys + Gly
-
 Homo sapiens ?
-
 ?