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Literature summary for 6.3.2.3 extracted from
- Glutathione synthetase from the fission yeast. Purification and its unique heteromeric subunit structure (1993), Biochem. Cell Biol., 71, 447-453.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | in presence of Mg2+ | Schizosaccharomyces pombe |  |
| Cd2+ | in presence of Mg2+ | Schizosaccharomyces pombe | |
| Cu2+ | in presence of Mg2+ | Schizosaccharomyces pombe | |
| Zn2+ | in presence of Mg2+ | Schizosaccharomyces pombe | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.27 | - |
gamma-Glu-Cys | - |
Schizosaccharomyces pombe | |
| 0.45 | - |
ATP | - |
Schizosaccharomyces pombe | |
| 0.67 | - |
Gly | - |
Schizosaccharomyces pombe | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| acetate | anions that favor activity in decreasing order: acetate, HCOO-, Cl-, at 50 mM K+ | Schizosaccharomyces pombe | |
| Cl- | anions that favor activity in decreasing order: acetate, HCOO-, Cl-, at 50 mM K+ | Schizosaccharomyces pombe | |
| HCOO- | anions that favor activity in decreasing order: acetate, HCOO-, Cl-, at 50 mM K+ | Schizosaccharomyces pombe | |
| K+ | strict requirement for monovalent cation, maximal activity with K+ | Schizosaccharomyces pombe | |
| K+ | maximal activation with 50 mM | Schizosaccharomyces pombe | |
| Li+ | can replace K+ | Schizosaccharomyces pombe | |
| Mg2+ | maximal activity at 20 mM | Schizosaccharomyces pombe | |
| Mn2+ | can partially replace Mg2+ in activation | Schizosaccharomyces pombe | |
| NH4+ | can replace K+ | Schizosaccharomyces pombe | |
| Zn2+ | can partially replace Mg2+ | Schizosaccharomyces pombe | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
2 * 33000 (alpha) + 2 * 26000 (beta), SDS-PAGE | Schizosaccharomyces pombe |
| 33000 | - |
2 * 33000 (alpha) + 2 * 26000 (beta), SDS-PAGE | Schizosaccharomyces pombe |
| 120000 | - |
gel filtration | Schizosaccharomyces pombe |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces pombe | - |
mutant MN101 transformed with pYS41 containing the glutathione synthetase gene | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | Gal-GlyNAc located at the terminal of the oligosaccharide chain | Schizosaccharomyces pombe |
| glycoprotein | contains fucose and mannose or glucose | Schizosaccharomyces pombe |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.05 | - |
- |
Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-Glu-L-Cys + Gly | - |
Schizosaccharomyces pombe | ADP + phosphate + glutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 33000 (alpha) + 2 * 26000 (beta), SDS-PAGE | Schizosaccharomyces pombe |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Schizosaccharomyces pombe |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Schizosaccharomyces pombe |
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Release 2023.1 (January 2023)
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