General Stability | Organism |
---|---|
several successive freezings and thawings of the cell-free extract have no effect on enzyme activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
gamma-Glu-Cys | above 2 mM | Escherichia coli | |
GSSG | no inhibition by reduced glutathione | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
Gly | - |
Escherichia coli | |
0.63 | - |
gamma-Glu-Cys | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-Glu-L-Cys + Gly | Escherichia coli | glutathione biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12, strain AB 1157 | - |
Storage Stability | Organism |
---|---|
-20°C, 20% loss of activity after 2 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-Glu-L-Cys + Gly | - |
Escherichia coli | ADP + phosphate + glutathione | i.e. gamma-Glu-Cys-Gly | ? | |
ATP + gamma-Glu-L-Cys + Gly | glutathione biosynthesis | Escherichia coli | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Escherichia coli |