Cloned (Comment) | Organism |
---|---|
Escherichia coli C600 cells transformed by a recombinant plasmid for the glutathione synthetase gene of Escherichia coli B | Escherichia coli |
General Stability | Organism |
---|---|
relatively stable at all stages of purification | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine | - |
Escherichia coli | |
ADP | - |
Escherichia coli | |
AMP | - |
Escherichia coli | |
GSSG | no inhibition by reduced glutathione | Escherichia coli | |
Hg2+ | - |
Escherichia coli | |
PCMB | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
ATP | - |
Escherichia coli | |
2 | - |
Gly | - |
Escherichia coli | |
2.6 | - |
gamma-Glu-Cys | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent metal ion required, Mg2+ is most effective, Co2+ can replace Mg2+ | Escherichia coli | |
Mg2+ | divalent metal ion required, Mg2+ is most effective | Escherichia coli | |
Mn2+ | can replace Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE | Escherichia coli |
152000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
B | - |
Escherichia coli B / ATCC 11303 | - |
B | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.83 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, stable for weeks | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-Glu-L-Cys + Gly | - |
Escherichia coli | ADP + phosphate + glutathione | - |
? | |
ATP + gamma-Glu-L-Cys + Gly | - |
Escherichia coli B / ATCC 11303 | ADP + phosphate + glutathione | - |
? | |
UTP + gamma-Glu-L-Cys + Gly | 10% of the activity relative to ATP | Escherichia coli | UDP + phosphate + glutathione | - |
? | |
UTP + gamma-Glu-L-Cys + Gly | 10% of the activity relative to ATP | Escherichia coli B / ATCC 11303 | UDP + phosphate + glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable below | Escherichia coli |
60 | - |
15 min, 50% loss of activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | stable | Escherichia coli |