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Literature summary for 6.3.2.3 extracted from

  • Gushima, H.; Miya, T.; Murata, K.; Kimura, A.
    Purification and characterization of glutathione synthetase from Escherichia coli B (1983), J. Appl. Biochem., 5, 210-218.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli C600 cells transformed by a recombinant plasmid for the glutathione synthetase gene of Escherichia coli B Escherichia coli

General Stability

General Stability Organism
relatively stable at all stages of purification Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
adenosine
-
Escherichia coli
ADP
-
Escherichia coli
AMP
-
Escherichia coli
GSSG no inhibition by reduced glutathione Escherichia coli
Hg2+
-
Escherichia coli
PCMB
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
ATP
-
Escherichia coli
2
-
Gly
-
Escherichia coli
2.6
-
gamma-Glu-Cys
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent metal ion required, Mg2+ is most effective, Co2+ can replace Mg2+ Escherichia coli
Mg2+ divalent metal ion required, Mg2+ is most effective Escherichia coli
Mn2+ can replace Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
x * 38000, SDS-PAGE Escherichia coli
152000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
B
-
Escherichia coli B / ATCC 11303
-
B
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.83
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, stable for weeks Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gamma-Glu-L-Cys + Gly
-
Escherichia coli ADP + phosphate + glutathione
-
?
ATP + gamma-Glu-L-Cys + Gly
-
Escherichia coli B / ATCC 11303 ADP + phosphate + glutathione
-
?
UTP + gamma-Glu-L-Cys + Gly 10% of the activity relative to ATP Escherichia coli UDP + phosphate + glutathione
-
?
UTP + gamma-Glu-L-Cys + Gly 10% of the activity relative to ATP Escherichia coli B / ATCC 11303 UDP + phosphate + glutathione
-
?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
stable below Escherichia coli
60
-
15 min, 50% loss of activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7.5 stable Escherichia coli