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Literature summary for 6.3.2.29 extracted from
- Isolation and characterization of a novel cyanophycin synthetase from a deep-sea sediment metagenomic library (2013), Appl. Microbiol. Biotechnol., 97, 8619-8628.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme requires a sulfhydryl component | uncultured bacterium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cphA49 cloned from deep-sea sediment metagenomic library with degenerated primers, amplified from the fss49 fosmid by PCR, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of His-tagged cphA49 in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | uncultured bacterium |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | cyanophycin is deposited in the cytoplasm asmembraneless granules | uncultured bacterium | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | required | uncultured bacterium |  |
| Mg2+ | required | uncultured bacterium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 98000 | - |
x * 98000, about, sequence calculation, x * 100000, recombinant His-tagged enzyme, SDS-PAGE | uncultured bacterium |
| 100000 | - |
x * 98000, about, sequence calculation, x * 100000, recombinant His-tagged enzyme, SDS-PAGE | uncultured bacterium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [L-Asp(4-L-Arg)]n + L-Asp | uncultured bacterium | - |
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp | - |
? | |
| additional information | uncultured bacterium | cyanophycin synthetase catalyzes the synthesis of cyanophycin by ATP-dependent polymerization of L-Asp and L-Arg. In vitro, the activity of CphA generally depends on the presence of L-Asp, L-Arg, ATP, Mg2+, K+, sulfhydryl compound, and cyanophycin as primers | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | M9UYB0 | gene cphA49 from deep-sea sediment metagenomic library | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged CphA49 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration | uncultured bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [L-Asp(4-L-Arg)]n + L-Asp | - |
uncultured bacterium | ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp | - |
? | |
| additional information | cyanophycin synthetase catalyzes the synthesis of cyanophycin by ATP-dependent polymerization of L-Asp and L-Arg. In vitro, the activity of CphA generally depends on the presence of L-Asp, L-Arg, ATP, Mg2+, K+, sulfhydryl compound, and cyanophycin as primers | uncultured bacterium | ? | - |
? | |
| additional information | the recombinant CphA49 exhibits strict primer dependency and broad substrate specificities. L-Lys and L-Glu can substitute for L-Asp, but with very low catalytic activity. No activity with L-Arg and L-citrulline, L-Arg and L-ornithine, L-Asp and L-citrulline, and L-Asp and L-ornithine | uncultured bacterium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 98000, about, sequence calculation, x * 100000, recombinant His-tagged enzyme, SDS-PAGE | uncultured bacterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CphA | - |
uncultured bacterium |
| CphA49 | - |
uncultured bacterium |
| cyanophycin synthetase | - |
uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
recombinant enzyme | uncultured bacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 70 | activity range, profile overview | uncultured bacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant enzyme, stable up to | uncultured bacterium |
| 50 | - |
purified recombinant enzyme, 60 min, over 8% activity remaining | uncultured bacterium |
| 70 | - |
purified recombinant enzyme, 15 min, inactivation | uncultured bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
recombinant enzyme | uncultured bacterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | activity range, high activity at a pH range of pH 8.0 to pH 10.5, recombinant enzyme, profile overview | uncultured bacterium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
uncultured bacterium | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | cyanophycin synthetase CphA49 belongs to NOR5 clade of Gammaproteobacteria enzymes | uncultured bacterium |
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