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Literature summary for 6.3.2.29 extracted from

  • Abd-El-Karem, Y.; Elbers, T.; Reichelt, R.; Steinbuechel, A.
    Heterologous expression of Anabaena sp. PCC7120 cyanophycin metabolism genes cphA1 and cphB1 in Sinorhizobium (Ensifer) meliloti 1021 (2011), Appl. Microbiol. Biotechnol., 89, 1177-1192.
    View publication on PubMed

Application

Application Comment Organism
synthesis expression of cyanophycin synthase in wild-type Sinorhizobium meliloti 1021 and in a phbC-negative mutant. Yeast mannitol broth yields the highest cyanophycin contents in both Sinorhizobium meliloti 1021 strains. Supplying the medium with isopropyl-beta-D-thiogalactopyranoside, aspartic acid, and arginine enhances cyanophycin contents about 2.5- and 2.8fold. Varying the nitrogen-to-carbon ratio in the medium enhanced the cyanophycin content further to 43.8% w/w of cell dry weight. Cyanophycin from the Sinorhizobium meliloti strains consists of equimolar amounts of aspartic acid and arginine and contains no other amino acids even if the medium is supplemented with glutamic acid, citrulline, ornithine, or lysine. Cyanophycin isolated from Sinorhizobium meliloti exhibits average molecular weights between 20 and 25 kDa. Cyanophycin isolated after expression in Escherichia coli S17-1 exhibits average molecular weight between 22 and 30 kDa. Co-expression of cyanophycinase from Anabaena sp. PCC7120 encoded by cphB17120 in cphA17120-positive Escherichia coli S17-1, Sinorhizobium meliloti 1021, and its phbC-negative mutant gives cyanophycinase activities in crude extracts, and no CGP granules occur Anabaena sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in the wild-type Sinorhizobium meliloti 1021 and in a phbC-negative mutant Anabaena sp.

Organism

Organism UniProt Comment Textmining
Anabaena sp.
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Anabaena sp. PCC 7120
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