Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.29 extracted from

  • Hai, T.; Lee, J.S.; Kim, T.J.; Suh, J.W.
    The role of the C-terminal region of cyanophycin synthetase from Nostoc ellipsosporum NE1 in its enzymatic activity and thermostability: A key function of Glu(856) (2008), Biochim. Biophys. Acta, 1794, 42-49.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene cphA, subcloning and expression of His-tagged wild-type and mutant enzymes in Escherichia coli strains DH5alpha and BL21(DE3) Nostoc ellipsosporum

Protein Variants

Protein Variants Comment Organism
E856A site-directed mutagenesis, inactive mutant Nostoc ellipsosporum
E856V site-directed mutagenesis, inactive mutant Nostoc ellipsosporum
additional information construction of truncation mutant CphANE1?C45, which lacks up to 45 amino acids at its C-terminus, the mutant retains full enzymatic activity and produced polymers, however, the removal of one additional amino acid, Glu856, results in complete inactivation of CphANE1?C46, phenotype, overview. Removal of the sequence Leu867-Leu870 results in dramatically decreased thermostability Nostoc ellipsosporum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Nostoc ellipsosporum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
210000
-
gel filtration, recombinant wild-type enzyme Nostoc ellipsosporum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n + L-Asp Nostoc ellipsosporum
-
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp Nostoc ellipsosporum NE1
-
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
?

Organism

Organism UniProt Comment Textmining
Nostoc ellipsosporum
-
-
-
Nostoc ellipsosporum NE1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Nostoc ellipsosporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n + L-Asp
-
Nostoc ellipsosporum ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp role of the C-terminal region of CphANE1, Glu856 is critical for CphANE1 catalytic activity, overview Nostoc ellipsosporum ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
-
Nostoc ellipsosporum NE1 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
?
additional information the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29 Nostoc ellipsosporum ?
-
?
additional information the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29 Nostoc ellipsosporum NE1 ?
-
?

Subunits

Subunits Comment Organism
dimer wild-type and truncation mutant CphANE1?C45 Nostoc ellipsosporum

Synonyms

Synonyms Comment Organism
CphA
-
Nostoc ellipsosporum
cyanophycin synthetase
-
Nostoc ellipsosporum
More cf. EC 6.3.2.30 Nostoc ellipsosporum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the predicted alpha-helical region that ranges from Val858 to Leu870 is important for the thermostability of the enzyme Nostoc ellipsosporum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Nostoc ellipsosporum