Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.29 extracted from

  • Berg, H.; Ziegler, K.; Piotukh, K.; Baier, K.; Lockau, W.; Volkmer-Engert, R.
    Biosynthesis of the cyanobacterial reserve polymer multi-L-arginyl-poly-L-aspartic acid (cyanophycin) (2000), Eur. J. Biochem., 267, 5561-5570.
    View publication on PubMed
Search for more literature for 6.3.2.29

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Trichormus variabilis ATCC 29413

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 L-Arg 28°C, pH 8.2 Trichormus variabilis ATCC 29413

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[L-Asp(4-L-Arg)]n + L-Asp + ATP Trichormus variabilis ATCC 29413 a small amount of cyanophycin is required as a primer [L-Asp(4-L-Arg)]n-Asp + ADP + phosphate no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase, EC 6.3.2.30 ?

Organism

Organism UniProt Comment Textmining
Trichormus variabilis ATCC 29413
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trichormus variabilis ATCC 29413

Reaction

Reaction Comment Organism Reaction ID
ATP + [L-Asp(4-L-Arg)]n + L-Asp = ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp i.e. the second reaction of cyanophycin synthesis catalysed by cyanophycin synthase, the first reaction is catalysed by the enzyme's other active centre Trichormus variabilis ATCC 29413
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]3-L-Asp + L-Arg
-
 Trichormus variabilis ATCC 29413 ADP + phosphate + [L-Asp(4-L-Arg)]4 + L-Asp
-
 ?
ATP + [L-Asp(4-L-Arg)]n + L-Asp [L-Asp(4-L-Arg)]n-Asp is a cyanophycin molecule with a C-terminal L-Asp residue that is not linked to an L-Arg residue via its beta-carboxy group, this intermediate is produced in the first reaction catalysed by cyanophycin synthase Trichormus variabilis ATCC 29413 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp no formation of AMP ?
L-aspartic acid + ATP
-
 Trichormus variabilis ATCC 29413 poly-L-aspartic acid + ADP + phosphate
-
 ?
additional information no product is formed if the peptide primer is blocked at the C-terminus or if L-arginine is solely supplied as substrate Trichormus variabilis ATCC 29413 ?
-
 ?
[L-Asp(4-L-Arg)]n + L-Asp + ATP a small amount of cyanophycin is required as a primer Trichormus variabilis ATCC 29413 [L-Asp(4-L-Arg)]n-Asp + ADP + phosphate no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase, EC 6.3.2.30 ?

Synonyms

Synonyms Comment Organism
CphA
-
 Trichormus variabilis ATCC 29413
cyanophycin synthetase
-
 Trichormus variabilis ATCC 29413