Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichoa coli | Thermococcus kodakarensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-phosphopantoate | substrate inhibition | Thermococcus kodakarensis | |
ATP | substrate inhibition | Thermococcus kodakarensis | |
additional information | feedback inhibition by CoA/acetyl-CoA and product inhibition by 4'-phosphopantothenate are not observed | Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
beta-Alanine | pH 6.5, 85°C | Thermococcus kodakarensis | |
1.16 | - |
(R)-4-phosphopantoate | pH 6.5, 85°C | Thermococcus kodakarensis | |
2.44 | - |
ATP | pH 6.5, 85°C | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-4-phosphopantoate + beta-alanine | Thermococcus kodakarensis | the enzyme is involved in coenzyme A biosynthesis in the archaea | AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q5JIZ8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.38 | - |
beta-alanine, pH 6.5, 85°C | Thermococcus kodakarensis |
14.7 | - |
ATP, pH 6.5, 85°C | Thermococcus kodakarensis |
15.6 | - |
(R)-4-phosphopantoate, pH 6.5, 85°C | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-4-phosphopantoate + beta-alanine | the enzyme is involved in coenzyme A biosynthesis in the archaea | Thermococcus kodakarensis | AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-4-phosphopantoate + beta-alanine | strict preference for ATP. Among several amine substrates, activity is detected with beta-alanine, but not with gamma-aminobutyrate, glycine nor aspartate | Thermococcus kodakarensis | AMP + diphosphate + (R)-4'-phosphopantothenate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphopantothenate synthetase | - |
Thermococcus kodakarensis |
Pps | - |
Thermococcus kodakarensis |
TK1686 | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
assay at | Thermococcus kodakarensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 95 | activity continues to increase with temperature elevation from 65°C up to 95°C | Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
6 h, no decrease in activity | Thermococcus kodakarensis |
80 | - |
half-life: 13.6 h | Thermococcus kodakarensis |
90 | - |
half-life: 8.2 h | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Thermococcus kodakarensis |