BRENDA - Enzyme Database show
show all sequences of 6.3.2.26

A detailed biochemical characterization of phosphopantothenate synthetase, a novel enzyme involved in coenzyme A biosynthesis in the Archaea

Ishibashi, T.; Tomita, H.; Yokooji, Y.; Morikita, T.; Watanabe, B.; Hiratake, J.; Kishimoto, A.; Kita, A.; Miki, K.; Imanaka, T.; Atomi, H.; Extremophiles 16, 819-828 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpressed in Escherichoa coli
Thermococcus kodakarensis
Inhibitors
Inhibitors
Commentary
Organism
Structure
4-phosphopantoate
substrate inhibition
Thermococcus kodakarensis
ATP
substrate inhibition
Thermococcus kodakarensis
additional information
feedback inhibition by CoA/acetyl-CoA and product inhibition by 4'-phosphopantothenate are not observed
Thermococcus kodakarensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.34
-
beta-Alanine
pH 6.5, 85°C
Thermococcus kodakarensis
1.16
-
(R)-4-phosphopantoate
pH 6.5, 85°C
Thermococcus kodakarensis
2.44
-
ATP
pH 6.5, 85°C
Thermococcus kodakarensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + (R)-4-phosphopantoate + beta-alanine
Thermococcus kodakarensis
the enzyme is involved in coenzyme A biosynthesis in the archaea
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermococcus kodakarensis
Q5JIZ8
-
-
Purification (Commentary)
Commentary
Organism
-
Thermococcus kodakarensis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.38
-
beta-alanine, pH 6.5, 85°C
Thermococcus kodakarensis
14.7
-
ATP, pH 6.5, 85°C
Thermococcus kodakarensis
15.6
-
(R)-4-phosphopantoate, pH 6.5, 85°C
Thermococcus kodakarensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (R)-4-phosphopantoate + beta-alanine
the enzyme is involved in coenzyme A biosynthesis in the archaea
727436
Thermococcus kodakarensis
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
-
?
ATP + (R)-4-phosphopantoate + beta-alanine
strict preference for ATP. Among several amine substrates, activity is detected with beta-alanine, but not with gamma-aminobutyrate, glycine nor aspartate
727436
Thermococcus kodakarensis
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
85
-
assay at
Thermococcus kodakarensis
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
65
95
activity continues to increase with temperature elevation from 65°C up to 95°C
Thermococcus kodakarensis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
6 h, no decrease in activity
Thermococcus kodakarensis
80
-
half-life: 13.6 h
Thermococcus kodakarensis
90
-
half-life: 8.2 h
Thermococcus kodakarensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Thermococcus kodakarensis
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpressed in Escherichoa coli
Thermococcus kodakarensis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-phosphopantoate
substrate inhibition
Thermococcus kodakarensis
ATP
substrate inhibition
Thermococcus kodakarensis
additional information
feedback inhibition by CoA/acetyl-CoA and product inhibition by 4'-phosphopantothenate are not observed
Thermococcus kodakarensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.34
-
beta-Alanine
pH 6.5, 85°C
Thermococcus kodakarensis
1.16
-
(R)-4-phosphopantoate
pH 6.5, 85°C
Thermococcus kodakarensis
2.44
-
ATP
pH 6.5, 85°C
Thermococcus kodakarensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + (R)-4-phosphopantoate + beta-alanine
Thermococcus kodakarensis
the enzyme is involved in coenzyme A biosynthesis in the archaea
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermococcus kodakarensis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.38
-
beta-alanine, pH 6.5, 85°C
Thermococcus kodakarensis
14.7
-
ATP, pH 6.5, 85°C
Thermococcus kodakarensis
15.6
-
(R)-4-phosphopantoate, pH 6.5, 85°C
Thermococcus kodakarensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (R)-4-phosphopantoate + beta-alanine
the enzyme is involved in coenzyme A biosynthesis in the archaea
727436
Thermococcus kodakarensis
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
-
?
ATP + (R)-4-phosphopantoate + beta-alanine
strict preference for ATP. Among several amine substrates, activity is detected with beta-alanine, but not with gamma-aminobutyrate, glycine nor aspartate
727436
Thermococcus kodakarensis
AMP + diphosphate + (R)-4'-phosphopantothenate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
85
-
assay at
Thermococcus kodakarensis
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
65
95
activity continues to increase with temperature elevation from 65°C up to 95°C
Thermococcus kodakarensis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
6 h, no decrease in activity
Thermococcus kodakarensis
80
-
half-life: 13.6 h
Thermococcus kodakarensis
90
-
half-life: 8.2 h
Thermococcus kodakarensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Thermococcus kodakarensis
Other publictions for EC 6.3.2.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744456
Suring
Biochemical pathways supporti ...
Folsomia candida
Biol. Open
5
1784-1789
2016
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1
1
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726783
Fernandez-Aguado
A vacuolar membrane protein af ...
Penicillium chrysogenum, Penicillium chrysogenum Wisconsin 54-1255
Appl. Microbiol. Biotechnol.
97
795-808
2013
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1
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1
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1
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2
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5
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2
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2
2
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727108
Wu
Motifs in the C-terminal regio ...
Penicillium chrysogenum, Penicillium chrysogenum Wisconsin 54-1255
Biochimie
94
354-364
2012
-
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1
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1
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1
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2
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5
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2
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1
1
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727436
Ishibashi
A detailed biochemical charact ...
Thermococcus kodakarensis
Extremophiles
16
819-828
2012
-
-
1
-
-
-
3
3
-
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1
-
4
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1
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3
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2
-
1
1
3
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1
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1
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3
-
3
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1
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1
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3
-
2
-
1
1
3
-
1
-
-
-
-
-
-
-
-
-
700004
Liras
Enzymology of beta-lactam comp ...
Actinomyces sp.
Methods Enzymol.
458
401-429
2009
-
1
1
-
-
-
4
-
-
-
4
3
-
5
-
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1
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3
1
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1
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1
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1
1
1
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4
-
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4
3
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1
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3
1
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1
-
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705518
Siewers
Heterologous production of non ...
Penicillium chrysogenum
Metab. Eng.
11
391-397
2009
-
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1
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1
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5
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706449
Gidijala
An engineered yeast efficientl ...
Penicillium chrysogenum
PLoS ONE
4
e8317
2009
-
1
1
-
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1
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6
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1
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1
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440074
Kallow
Penicillin biosynthesis: energ ...
Acremonium chrysogenum
Biochemistry
37
5947-5952
1998
-
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2
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2
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1
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1
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1
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-
1
1
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-
2
-
-
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-
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-
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440073
Kallow
Penicillin biosynthesis: inter ...
Acremonium chrysogenum
FEBS Lett.
414
74-78
1997
-
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2
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1
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1
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1
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1
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440080
Shiau
L-delta-(alpha-Aminoadipoyl)-L ...
Acremonium chrysogenum
Biochemistry
36
8798-8806
1997
-
-
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1
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1
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2
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440084
Theilgaard
-
Purification and characterizat ...
Penicillium chrysogenum
Biochem. J.
327
185-191
1997
-
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2
3
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1
1
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1
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1
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1
1
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2
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1
1
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1
1
1
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2
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440086
Byford
The mechanism of ACV synthetas ...
Acremonium chrysogenum, Aspergillus nidulans, Penicillium chrysogenum, Streptomyces clavuligerus
Chem. Rev.
97
2631-2649
1997
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1
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4
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4
2
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440082
Coque
Overexpression of the Nocardia ...
Amycolatopsis lactamdurans
Eur. J. Biochem.
242
264-270
1996
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1
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1
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1
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1
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3
1
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440079
Shiau
Molecular mechanism of the mul ...
Acremonium chrysogenum
Biochem. Soc. Trans.
23
629S
1995
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440077
Baldwin
-
Substrate specificity of L-del ...
Acremonium chrysogenum, Streptomyces clavuligerus
Biochem. J.
301
367-372
1994
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2
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11
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2
11
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440085
Aharonowitz
delta-(L-alpha-Aminoadipyl)-L- ...
Acremonium chrysogenum, Amycolatopsis lactamdurans, Aspergillus nidulans, Lysobacter lactamgenus, Paecilomyces persicinus, Penicillium chrysogenum
Bio/Technology
11
807-810
1993
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6
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440078
Zhang
-
Regulation of ACV synthetase a ...
Acremonium chrysogenum, Streptomyces clavuligerus
Arch. Microbiol.
158
364-369
1992
-
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5
-
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2
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2
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2
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2
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440081
Zhang
Biochemical studies on the act ...
Streptomyces clavuligerus
Biochem. J.
283
691-698
1992
-
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8
3
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1
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1
2
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1
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6
-
1
1
3
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1
1
2
1
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1
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8
-
3
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1
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1
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1
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6
-
1
1
3
-
1
1
2
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440083
Zhang
-
In vitro stabilization of ACV ...
Streptomyces clavuligerus
Appl. Biochem. Biotechnol.
37
97-110
1992
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440075
MacCabe
delta-(L-alpha-Aminoadipyl)-L- ...
Aspergillus nidulans
J. Biol. Chem.
266
12646-12654
1991
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440076
Banko
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delta-(L-alpha-Aminoadipyl)-L- ...
Acremonium chrysogenum
J. Am. Chem. Soc.
109
2858-2860
1987
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