BRENDA - Enzyme Database show
show all sequences of 6.3.2.26

Enzymology of beta-lactam compounds with cephem structure produced by actinomycete

Liras, P.; Demain, A.L.; Methods Enzymol. 458, 401-429 (2009)

Data extracted from this reference:

Application
Application
Commentary
Organism
biotechnology
production of beta-lactam antibiotics
Actinomyces sp.
Cloned(Commentary)
Commentary
Organism
ACVS of Streptomyces lactamdurans is produced as a recombinant protein from a Streptomyces lividans transformant, carrying the pcbAB gene
Actinomyces sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis-2-nitrobenzoate
the major effect on ACVS activity is exerted by thiol-blocking agents, which almost completely inhibited the activity at 1 mM concentration, confirming the importance of thiol groups in ACVS activity
Actinomyces sp.
iodoacetamide
the major effect on ACVS activity is exerted by thiol-blocking agents, which almost completely inhibited the activity at 1 mM concentration, confirming the importance of thiol groups in ACVS activity
Actinomyces sp.
N-ethylmaleimide
the major effect on ACVS activity is exerted by thiol-blocking agents, which almost completely inhibited the activity at 1 mM concentration, confirming the importance of thiol groups in ACVS activity
Actinomyces sp.
phosphate
ACVS activity decreases 30% in the presence of 100 mM phosphate, being also affected by AMP and pyrophosphate, products of ATP hydrolysis
Actinomyces sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
subunit of Streptomyces clavuligerus ACVS
Actinomyces sp.
283000
-
subunit of Streptomyces clavuligerus ACVS
Actinomyces sp.
404100
-
pcbAB gene from Nocardia lactamdurans is 10.9 kb long and encodes a 3649 amino acid ACVS
Actinomyces sp.
560000
-
monomer of Streptomyces clavuligerus ACVS that appears as a 500kDa band in SDS-PAGE
Actinomyces sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-aminoadipic acid + L-valine + L-lysine + ?
Actinomyces sp.
enzyme is a nonribosomal peptide synthetase (NRPS) able to form ribosome-independent peptide bonds
L-delta-alpha-aminoadipyl-L-cysteinyl-D-valine + ?
ACV
-
?
additional information
Actinomyces sp.
ACVSs are multifunctional enzymes that activate amino acids as aminoacyladenylates, forming a mixed anhydride with the a-phosphate group of ATP and releasing pyrophosphate
?
-
-
-
additional information
Actinomyces sp.
pure Streptomyces lactamdurans ACVS is able to activate alpha-aminoadipic acid or its lactam 6-oxopiperideine-2-carboxylic acid, a compound that is easily converted to alpha-aminoadipic acid, but is unable to use piperideine-6-carboxylate or pipecolic acid as substrates, enzyme is also able to use L-cystathionine with the same efficiency as L-cysteine
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Actinomyces sp.
-
-
-
Purification (Commentary)
Commentary
Organism
ACVS of Streptomyces clavuligerus is purified 12fold using a combination of 2 successive chromatography steps on MonoQ columns separated by ultrafiltration; the recombinant of Streptomyces clavuligerus ACVS is purified from a Streptomyces lividans transformant 2785fold to near homogeneity by a combination of gel filtration, ultrafiltration, and ion-exchange chromatography
Actinomyces sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-aminoadipic acid + L-valine + L-lysine + ?
enzyme is a nonribosomal peptide synthetase (NRPS) able to form ribosome-independent peptide bonds
700004
Actinomyces sp.
L-delta-alpha-aminoadipyl-L-cysteinyl-D-valine + ?
ACV
-
-
?
additional information
ACVSs are multifunctional enzymes that activate amino acids as aminoacyladenylates, forming a mixed anhydride with the a-phosphate group of ATP and releasing pyrophosphate
700004
Actinomyces sp.
?
-
-
-
-
additional information
pure Streptomyces lactamdurans ACVS is able to activate alpha-aminoadipic acid or its lactam 6-oxopiperideine-2-carboxylic acid, a compound that is easily converted to alpha-aminoadipic acid, but is unable to use piperideine-6-carboxylate or pipecolic acid as substrates, enzyme is also able to use L-cystathionine with the same efficiency as L-cysteine
700004
Actinomyces sp.
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
heterodimer
ACVS from Streptomyces clavuligerus 2 subunits of 283 kDa and a subunit of 32 kDa
Actinomyces sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
8.5
optimal pH-range for ACVS
Actinomyces sp.
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
ACVS has a adenylate-containing modules for ATP binding
Actinomyces sp.
Application (protein specific)
Application
Commentary
Organism
biotechnology
production of beta-lactam antibiotics
Actinomyces sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
ACVS of Streptomyces lactamdurans is produced as a recombinant protein from a Streptomyces lividans transformant, carrying the pcbAB gene
Actinomyces sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
ACVS has a adenylate-containing modules for ATP binding
Actinomyces sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis-2-nitrobenzoate
the major effect on ACVS activity is exerted by thiol-blocking agents, which almost completely inhibited the activity at 1 mM concentration, confirming the importance of thiol groups in ACVS activity
Actinomyces sp.
iodoacetamide
the major effect on ACVS activity is exerted by thiol-blocking agents, which almost completely inhibited the activity at 1 mM concentration, confirming the importance of thiol groups in ACVS activity
Actinomyces sp.
N-ethylmaleimide
the major effect on ACVS activity is exerted by thiol-blocking agents, which almost completely inhibited the activity at 1 mM concentration, confirming the importance of thiol groups in ACVS activity
Actinomyces sp.
phosphate
ACVS activity decreases 30% in the presence of 100 mM phosphate, being also affected by AMP and pyrophosphate, products of ATP hydrolysis
Actinomyces sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
subunit of Streptomyces clavuligerus ACVS
Actinomyces sp.
283000
-
subunit of Streptomyces clavuligerus ACVS
Actinomyces sp.
404100
-
pcbAB gene from Nocardia lactamdurans is 10.9 kb long and encodes a 3649 amino acid ACVS
Actinomyces sp.
560000
-
monomer of Streptomyces clavuligerus ACVS that appears as a 500kDa band in SDS-PAGE
Actinomyces sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-aminoadipic acid + L-valine + L-lysine + ?
Actinomyces sp.
enzyme is a nonribosomal peptide synthetase (NRPS) able to form ribosome-independent peptide bonds
L-delta-alpha-aminoadipyl-L-cysteinyl-D-valine + ?
ACV
-
?
additional information
Actinomyces sp.
ACVSs are multifunctional enzymes that activate amino acids as aminoacyladenylates, forming a mixed anhydride with the a-phosphate group of ATP and releasing pyrophosphate
?
-
-
-
additional information
Actinomyces sp.
pure Streptomyces lactamdurans ACVS is able to activate alpha-aminoadipic acid or its lactam 6-oxopiperideine-2-carboxylic acid, a compound that is easily converted to alpha-aminoadipic acid, but is unable to use piperideine-6-carboxylate or pipecolic acid as substrates, enzyme is also able to use L-cystathionine with the same efficiency as L-cysteine
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
ACVS of Streptomyces clavuligerus is purified 12fold using a combination of 2 successive chromatography steps on MonoQ columns separated by ultrafiltration; the recombinant of Streptomyces clavuligerus ACVS is purified from a Streptomyces lividans transformant 2785fold to near homogeneity by a combination of gel filtration, ultrafiltration, and ion-exchange chromatography
Actinomyces sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-aminoadipic acid + L-valine + L-lysine + ?
enzyme is a nonribosomal peptide synthetase (NRPS) able to form ribosome-independent peptide bonds
700004
Actinomyces sp.
L-delta-alpha-aminoadipyl-L-cysteinyl-D-valine + ?
ACV
-
-
?
additional information
ACVSs are multifunctional enzymes that activate amino acids as aminoacyladenylates, forming a mixed anhydride with the a-phosphate group of ATP and releasing pyrophosphate
700004
Actinomyces sp.
?
-
-
-
-
additional information
pure Streptomyces lactamdurans ACVS is able to activate alpha-aminoadipic acid or its lactam 6-oxopiperideine-2-carboxylic acid, a compound that is easily converted to alpha-aminoadipic acid, but is unable to use piperideine-6-carboxylate or pipecolic acid as substrates, enzyme is also able to use L-cystathionine with the same efficiency as L-cysteine
700004
Actinomyces sp.
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
heterodimer
ACVS from Streptomyces clavuligerus 2 subunits of 283 kDa and a subunit of 32 kDa
Actinomyces sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
8.5
optimal pH-range for ACVS
Actinomyces sp.
Other publictions for EC 6.3.2.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744456
Suring
Biochemical pathways supporti ...
Folsomia candida
Biol. Open
5
1784-1789
2016
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1
1
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726783
Fernandez-Aguado
A vacuolar membrane protein af ...
Penicillium chrysogenum, Penicillium chrysogenum Wisconsin 54-1255
Appl. Microbiol. Biotechnol.
97
795-808
2013
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1
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5
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2
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727108
Wu
Motifs in the C-terminal regio ...
Penicillium chrysogenum, Penicillium chrysogenum Wisconsin 54-1255
Biochimie
94
354-364
2012
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1
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727436
Ishibashi
A detailed biochemical charact ...
Thermococcus kodakarensis
Extremophiles
16
819-828
2012
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1
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3
3
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1
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2
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1
1
3
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1
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700004
Liras
Enzymology of beta-lactam comp ...
Actinomyces sp.
Methods Enzymol.
458
401-429
2009
-
1
1
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4
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4
3
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5
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1
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3
1
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1
1
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4
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3
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1
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1
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1
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705518
Siewers
Heterologous production of non ...
Penicillium chrysogenum
Metab. Eng.
11
391-397
2009
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-
1
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1
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5
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706449
Gidijala
An engineered yeast efficientl ...
Penicillium chrysogenum
PLoS ONE
4
e8317
2009
-
1
1
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1
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6
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440074
Kallow
Penicillin biosynthesis: energ ...
Acremonium chrysogenum
Biochemistry
37
5947-5952
1998
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2
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2
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1
1
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2
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440073
Kallow
Penicillin biosynthesis: inter ...
Acremonium chrysogenum
FEBS Lett.
414
74-78
1997
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440080
Shiau
L-delta-(alpha-Aminoadipoyl)-L ...
Acremonium chrysogenum
Biochemistry
36
8798-8806
1997
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1
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440084
Theilgaard
-
Purification and characterizat ...
Penicillium chrysogenum
Biochem. J.
327
185-191
1997
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2
3
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1
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1
1
1
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2
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440086
Byford
The mechanism of ACV synthetas ...
Acremonium chrysogenum, Aspergillus nidulans, Penicillium chrysogenum, Streptomyces clavuligerus
Chem. Rev.
97
2631-2649
1997
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2
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440082
Coque
Overexpression of the Nocardia ...
Amycolatopsis lactamdurans
Eur. J. Biochem.
242
264-270
1996
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1
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1
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440079
Shiau
Molecular mechanism of the mul ...
Acremonium chrysogenum
Biochem. Soc. Trans.
23
629S
1995
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440077
Baldwin
-
Substrate specificity of L-del ...
Acremonium chrysogenum, Streptomyces clavuligerus
Biochem. J.
301
367-372
1994
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11
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2
11
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1
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440085
Aharonowitz
delta-(L-alpha-Aminoadipyl)-L- ...
Acremonium chrysogenum, Amycolatopsis lactamdurans, Aspergillus nidulans, Lysobacter lactamgenus, Paecilomyces persicinus, Penicillium chrysogenum
Bio/Technology
11
807-810
1993
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6
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440078
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