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Literature summary for 6.3.2.25 extracted from
- Enzyme which specifically adds tyrosine to the alpha chain of tubulin (1977), Biochemistry, 16, 2189-2194.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-L-Glu-L-Tyr | - |
Bos taurus |  |
| L-Ala-L-Tyr | - |
Bos taurus | |
| L-hydroxyphenylalanine | - |
Bos taurus | |
| L-Phe | - |
Bos taurus | |
| L-Tyr-Gly | - |
Bos taurus | |
| L-Tyr-L-Ala | - |
Bos taurus | |
| L-Tyr-L-Glu | - |
Bos taurus | |
| L-Tyr-L-Tyr | - |
Bos taurus | |
| tyramine | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
alpha-tubulin | below 0.002 mM | Bos taurus | |
| 0.045 | - |
L-Tyr | - |
Bos taurus | |
| 0.16 | - |
L-hydroxyphenylalanine | - |
Bos taurus | |
| 2.9 | - |
L-Phe | - |
Bos taurus | |
| 8 | - |
tyramine | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
gel filtration, MW of enzyme in crude extract, the MW of the purified enzyme is 150000. Addition of pure dimeric tubulin to the 35000 MW enzyme converts it back to the larger form which is apparently a stoichiometric 1:1 complex of tubulin and the 35000 MW enzyme | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + detyrosinated alpha-tubulin + L-Tyr | Bos taurus | posttranslational modification of tubulin with potential significance for the regulation of microtubule assembly or function | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.039 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-tubulin + ADP + phosphate | - |
Bos taurus | ATP + detyrosinated alpha-tubulin + L-tyrosine | - |
r | |
| ATP + detyrosinated alpha-tubulin + L-hydroxyphenylalanine | - |
Bos taurus | ? | - |
? | |
| ATP + detyrosinated alpha-tubulin + L-Phe | - |
Bos taurus | ? | - |
? | |
| ATP + detyrosinated alpha-tubulin + L-Tyr | posttranslational modification of tubulin with potential significance for the regulation of microtubule assembly or function | Bos taurus | ? | - |
? | |
| ATP + detyrosinated alpha-tubulin + tyramine | - |
Bos taurus | ? | - |
? | |
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Release 2023.1 (January 2023)
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