BRENDA - Enzyme Database show
show all sequences of 6.3.2.24

Tyrosyl-tRNA synthetase A potential kyotorphin synthetase in mammals

Tsukahara, T.; Yamagishi, S.; Neyama, H.; Ueda, H.; Peptides 101, 60-68 (2018)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli; real-time PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Homo sapiens
0.2
-
L-tyrosine
pH 7.4, 37°C; pH 7.4, 37°C, recombinant His-tagged enzyme
Homo sapiens
1.4
-
L-arginine
pH 7.4, 37°C; pH 7.4, 37°C, recombinant His-tagged enzyme
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required. Assay in presence of 100 mM
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-
Mus musculus C57BL/6
-
-
-
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adrenal gland
-
Homo sapiens
-
amygdala
-
Mus musculus
-
brain
-
Homo sapiens
-
brain
expression pattern in mouse brain of TyrRS and KTP synthesis after Arg administration
Mus musculus
-
brain
-
Rattus norvegicus
-
cerebellum
-
Mus musculus
-
cerebral cortex
-
Mus musculus
-
hippocampus
-
Mus musculus
-
hypothalamus
-
Mus musculus
-
medulla oblongata
; TyrRS levels are greater in the midbrain and medulla oblongata than in other brain regions. When arginine is administered 2 h prior to brain dissection, the kyotorphin levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even
Mus musculus
-
midbrain
; TyrRS levels are greater in the midbrain and medulla oblongata than in other brain regions. When arginine is administered 2 h prior to brain dissection, the kyotorphin levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even
Mus musculus
-
additional information
expression in all organs tested
Homo sapiens
-
olfactory bulb
-
Mus musculus
-
pancreas
; highest expression level
Homo sapiens
-
PC-12 cell
-
Rattus norvegicus
-
skeletal muscle
-
Homo sapiens
-
striatum
-
Mus musculus
-
testis
-
Homo sapiens
-
thalamus
-
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + L-tyrosine + L-arginine
-
745971
Mus musculus
AMP + diphosphate + L-tyrosyl-L-arginine
-
-
-
?
ATP + L-tyrosine + L-arginine
-
745971
Homo sapiens
AMP + diphosphate + L-tyrosyl-L-arginine
-
-
-
?
ATP + L-tyrosine + L-arginine
-
745971
Rattus norvegicus
AMP + diphosphate + L-tyrosyl-L-arginine
-
-
-
?
ATP + L-tyrosine + L-arginine
-
745971
Mus musculus C57BL/6
AMP + diphosphate + L-tyrosyl-L-arginine
-
-
-
?
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Mus musculus
?
-
-
-
-
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Homo sapiens
?
-
-
-
-
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Rattus norvegicus
?
-
-
-
-
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Mus musculus C57BL/6
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 59000, recombinant His6-tagged enzyme, SDS-PAGE
Homo sapiens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Mus musculus
37
-
assay at
Rattus norvegicus
50
-
-
Homo sapiens
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
37
70
60% of maximal activity at 37°C , 30% at 70°C , maximal activity at 50°C, no activity at 100°C
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
-
Homo sapiens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
2.2
9.5
KTP production is highest at pH 7.4, and is approximately half of that at pH 9.5. Production is markedly lower under acidic conditions at pH 3.8 and pH 2.2
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Homo sapiens
ATP
-
Mus musculus
ATP
-
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli; real-time PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Homo sapiens
ATP
-
Mus musculus
ATP
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Homo sapiens
0.2
-
L-tyrosine
pH 7.4, 37°C; pH 7.4, 37°C, recombinant His-tagged enzyme
Homo sapiens
1.4
-
L-arginine
pH 7.4, 37°C; pH 7.4, 37°C, recombinant His-tagged enzyme
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required. Assay in presence of 100 mM
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adrenal gland
-
Homo sapiens
-
amygdala
-
Mus musculus
-
brain
-
Homo sapiens
-
brain
expression pattern in mouse brain of TyrRS and KTP synthesis after Arg administration
Mus musculus
-
brain
-
Rattus norvegicus
-
cerebellum
-
Mus musculus
-
cerebral cortex
-
Mus musculus
-
hippocampus
-
Mus musculus
-
hypothalamus
-
Mus musculus
-
medulla oblongata
; TyrRS levels are greater in the midbrain and medulla oblongata than in other brain regions. When arginine is administered 2 h prior to brain dissection, the kyotorphin levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even
Mus musculus
-
midbrain
; TyrRS levels are greater in the midbrain and medulla oblongata than in other brain regions. When arginine is administered 2 h prior to brain dissection, the kyotorphin levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even
Mus musculus
-
additional information
expression in all organs tested
Homo sapiens
-
olfactory bulb
-
Mus musculus
-
pancreas
; highest expression level
Homo sapiens
-
PC-12 cell
-
Rattus norvegicus
-
skeletal muscle
-
Homo sapiens
-
striatum
-
Mus musculus
-
testis
-
Homo sapiens
-
thalamus
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + L-tyrosine + L-arginine
-
745971
Mus musculus
AMP + diphosphate + L-tyrosyl-L-arginine
-
-
-
?
ATP + L-tyrosine + L-arginine
-
745971
Homo sapiens
AMP + diphosphate + L-tyrosyl-L-arginine
-
-
-
?
ATP + L-tyrosine + L-arginine
-
745971
Rattus norvegicus
AMP + diphosphate + L-tyrosyl-L-arginine
-
-
-
?
ATP + L-tyrosine + L-arginine
-
745971
Mus musculus C57BL/6
AMP + diphosphate + L-tyrosyl-L-arginine
-
-
-
?
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Mus musculus
?
-
-
-
-
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Homo sapiens
?
-
-
-
-
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Rattus norvegicus
?
-
-
-
-
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Mus musculus C57BL/6
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 59000, recombinant His6-tagged enzyme, SDS-PAGE
Homo sapiens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Mus musculus
37
-
assay at
Rattus norvegicus
50
-
-
Homo sapiens
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
37
70
60% of maximal activity at 37°C , 30% at 70°C , maximal activity at 50°C, no activity at 100°C
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
-
Homo sapiens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
2.2
9.5
KTP production is highest at pH 7.4, and is approximately half of that at pH 9.5. Production is markedly lower under acidic conditions at pH 3.8 and pH 2.2
Homo sapiens
General Information
General Information
Commentary
Organism
malfunction
when arginine is administered 2 h prior to brain dissection, the KTP levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even
Mus musculus
malfunction
TyrRS knockdown of PC12 cells with a small interfering RNA (siRNA) in the presence of 1.6mM tyrosine, arginine, proline, or tryptophan significantly reduces the level of KTP, but not those of tyrosine-tyrosine, tyrosine-proline, or tyrosine-tryptophan. siRNA treatment does not affect cell survival or proliferation
Rattus norvegicus
physiological function
tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production; TyrRS knockdown of PC12 cells with siRNA in the presence of 1.6 mM tyrosine, arginine, proline, or tryptophan significantly reduces the level of product kyotorphin, but not those of tyrosine-tyrosine, tyrosine-proline, or tyrosine-tryptophan. siRNA treatment does not affect cell survival or proliferation
Homo sapiens
physiological function
tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production
Mus musculus
physiological function
tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production
Rattus norvegicus
General Information (protein specific)
General Information
Commentary
Organism
malfunction
when arginine is administered 2 h prior to brain dissection, the KTP levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even
Mus musculus
malfunction
TyrRS knockdown of PC12 cells with a small interfering RNA (siRNA) in the presence of 1.6mM tyrosine, arginine, proline, or tryptophan significantly reduces the level of KTP, but not those of tyrosine-tyrosine, tyrosine-proline, or tyrosine-tryptophan. siRNA treatment does not affect cell survival or proliferation
Rattus norvegicus
physiological function
tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production; TyrRS knockdown of PC12 cells with siRNA in the presence of 1.6 mM tyrosine, arginine, proline, or tryptophan significantly reduces the level of product kyotorphin, but not those of tyrosine-tyrosine, tyrosine-proline, or tyrosine-tryptophan. siRNA treatment does not affect cell survival or proliferation
Homo sapiens
physiological function
tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production
Mus musculus
physiological function
tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production
Rattus norvegicus
Other publictions for EC 6.3.2.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745971
Tsukahara
Tyrosyl-tRNA synthetase A pot ...
Homo sapiens, Mus musculus, Mus musculus C57BL/6, Rattus norvegicus
Peptides
101
60-68
2018
-
-
1
-
-
-
-
3
-
1
-
-
-
145
-
-
1
-
-
19
-
-
8
1
3
1
-
-
1
1
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
3
-
1
-
-
-
-
-
1
-
19
-
-
8
1
3
1
-
-
1
1
-
-
-
5
5
-
-
-
648948
Kawabata
Kyotorphin synthetase activity ...
Rattus norvegicus
Peptides
17
407-411
1996
-
-
-
-
-
-
4
5
1
1
1
-
-
2
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
5
1
1
1
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648949
Kawabata
NG-nitro-L-arginine methyl est ...
Rattus norvegicus
Peptides
16
1317-1319
1995
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648947
Ueda
Kyotorphin (tyrosine-arginine) ...
Rattus norvegicus
J. Biol. Chem.
262
8165-8173
1987
-
-
-
-
-
-
6
4
2
2
1
1
-
2
-
-
1
-
-
1
1
-
14
-
1
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
6
-
4
2
2
1
1
-
-
-
1
-
1
1
-
14
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-