Data extracted from this reference:
Cloned(Commentary)
expression in Escherichia coli; real-time PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Homo sapiens
KM Value [mM]
additional information
additional information
Michaelis-Menten kinetics
Homo sapiens
0.2
L-tyrosine
pH 7.4, 37°C; pH 7.4, 37°C, recombinant His-tagged enzyme
Homo sapiens
1.4
L-arginine
pH 7.4, 37°C; pH 7.4, 37°C, recombinant His-tagged enzyme
Homo sapiens
Metals/Ions
Mg2+
required. Assay in presence of 100 mM
Homo sapiens
Organism
Purification (Commentary)
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Homo sapiens
Source Tissue
adrenal gland
Homo sapiens
brain
expression pattern in mouse brain of TyrRS and KTP synthesis after Arg administration
Mus musculus
cerebral cortex
Mus musculus
hypothalamus
Mus musculus
medulla oblongata
; TyrRS levels are greater in the midbrain and medulla oblongata than in other brain regions. When arginine is administered 2 h prior to brain dissection, the kyotorphin levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even
Mus musculus
midbrain
; TyrRS levels are greater in the midbrain and medulla oblongata than in other brain regions. When arginine is administered 2 h prior to brain dissection, the kyotorphin levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even
Mus musculus
additional information
expression in all organs tested
Homo sapiens
olfactory bulb
Mus musculus
pancreas
; highest expression level
Homo sapiens
PC-12 cell
Rattus norvegicus
skeletal muscle
Homo sapiens
Substrates and Products (Substrate)
ATP + L-tyrosine + L-arginine
745971
Mus musculus
AMP + diphosphate + L-tyrosyl-L-arginine
?
ATP + L-tyrosine + L-arginine
745971
Homo sapiens
AMP + diphosphate + L-tyrosyl-L-arginine
?
ATP + L-tyrosine + L-arginine
745971
Rattus norvegicus
AMP + diphosphate + L-tyrosyl-L-arginine
?
ATP + L-tyrosine + L-arginine
745971
Mus musculus C57BL/6
AMP + diphosphate + L-tyrosyl-L-arginine
?
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Mus musculus
?
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Homo sapiens
?
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Rattus norvegicus
?
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Mus musculus C57BL/6
?
Subunits
?
x * 59000, recombinant His6-tagged enzyme, SDS-PAGE
Homo sapiens
Synonyms
KTP synthetase
Homo sapiens
KTP synthetase
Mus musculus
KTP synthetase
Rattus norvegicus
kyotorphin synthetase
Homo sapiens
kyotorphin synthetase
Mus musculus
kyotorphin synthetase
Rattus norvegicus
Temperature Optimum [°C]
37
assay at
Rattus norvegicus
Temperature Range [°C]
37
70
60% of maximal activity at 37°C , 30% at 70°C , maximal activity at 50°C, no activity at 100°C
Homo sapiens
pH Optimum
pH Range
2.2
9.5
KTP production is highest at pH 7.4, and is approximately half of that at pH 9.5. Production is markedly lower under acidic conditions at pH 3.8 and pH 2.2
Homo sapiens
Cofactor
Cloned(Commentary) (protein specific)
expression in Escherichia coli; real-time PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Homo sapiens
Cofactor (protein specific)
KM Value [mM] (protein specific)
additional information
additional information
Michaelis-Menten kinetics
Homo sapiens
0.2
L-tyrosine
pH 7.4, 37°C; pH 7.4, 37°C, recombinant His-tagged enzyme
Homo sapiens
1.4
L-arginine
pH 7.4, 37°C; pH 7.4, 37°C, recombinant His-tagged enzyme
Homo sapiens
Metals/Ions (protein specific)
Mg2+
required. Assay in presence of 100 mM
Homo sapiens
Purification (Commentary) (protein specific)
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Homo sapiens
Source Tissue (protein specific)
adrenal gland
Homo sapiens
brain
expression pattern in mouse brain of TyrRS and KTP synthesis after Arg administration
Mus musculus
cerebral cortex
Mus musculus
hypothalamus
Mus musculus
medulla oblongata
; TyrRS levels are greater in the midbrain and medulla oblongata than in other brain regions. When arginine is administered 2 h prior to brain dissection, the kyotorphin levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even
Mus musculus
midbrain
; TyrRS levels are greater in the midbrain and medulla oblongata than in other brain regions. When arginine is administered 2 h prior to brain dissection, the kyotorphin levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even
Mus musculus
additional information
expression in all organs tested
Homo sapiens
olfactory bulb
Mus musculus
pancreas
; highest expression level
Homo sapiens
PC-12 cell
Rattus norvegicus
skeletal muscle
Homo sapiens
Substrates and Products (Substrate) (protein specific)
ATP + L-tyrosine + L-arginine
745971
Mus musculus
AMP + diphosphate + L-tyrosyl-L-arginine
?
ATP + L-tyrosine + L-arginine
745971
Homo sapiens
AMP + diphosphate + L-tyrosyl-L-arginine
?
ATP + L-tyrosine + L-arginine
745971
Rattus norvegicus
AMP + diphosphate + L-tyrosyl-L-arginine
?
ATP + L-tyrosine + L-arginine
745971
Mus musculus C57BL/6
AMP + diphosphate + L-tyrosyl-L-arginine
?
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Mus musculus
?
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Homo sapiens
?
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Rattus norvegicus
?
additional information
recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1
745971
Mus musculus C57BL/6
?
Subunits (protein specific)
?
x * 59000, recombinant His6-tagged enzyme, SDS-PAGE
Homo sapiens
Temperature Optimum [°C] (protein specific)
37
assay at
Rattus norvegicus
Temperature Range [°C] (protein specific)
37
70
60% of maximal activity at 37°C , 30% at 70°C , maximal activity at 50°C, no activity at 100°C
Homo sapiens
pH Optimum (protein specific)
pH Range (protein specific)
2.2
9.5
KTP production is highest at pH 7.4, and is approximately half of that at pH 9.5. Production is markedly lower under acidic conditions at pH 3.8 and pH 2.2
Homo sapiens
General Information
malfunction
when arginine is administered 2 h prior to brain dissection, the KTP levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even
Mus musculus
malfunction
TyrRS knockdown of PC12 cells with a small interfering RNA (siRNA) in the presence of 1.6mM tyrosine, arginine, proline, or tryptophan significantly reduces the level of KTP, but not those of tyrosine-tyrosine, tyrosine-proline, or tyrosine-tryptophan. siRNA treatment does not affect cell survival or proliferation
Rattus norvegicus
physiological function
tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production; TyrRS knockdown of PC12 cells with siRNA in the presence of 1.6 mM tyrosine, arginine, proline, or tryptophan significantly reduces the level of product kyotorphin, but not those of tyrosine-tyrosine, tyrosine-proline, or tyrosine-tryptophan. siRNA treatment does not affect cell survival or proliferation
Homo sapiens
physiological function
tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production
Mus musculus
physiological function
tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production
Rattus norvegicus
General Information (protein specific)
malfunction
when arginine is administered 2 h prior to brain dissection, the KTP levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even
Mus musculus
malfunction
TyrRS knockdown of PC12 cells with a small interfering RNA (siRNA) in the presence of 1.6mM tyrosine, arginine, proline, or tryptophan significantly reduces the level of KTP, but not those of tyrosine-tyrosine, tyrosine-proline, or tyrosine-tryptophan. siRNA treatment does not affect cell survival or proliferation
Rattus norvegicus
physiological function
tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production; TyrRS knockdown of PC12 cells with siRNA in the presence of 1.6 mM tyrosine, arginine, proline, or tryptophan significantly reduces the level of product kyotorphin, but not those of tyrosine-tyrosine, tyrosine-proline, or tyrosine-tryptophan. siRNA treatment does not affect cell survival or proliferation
Homo sapiens
physiological function
tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production
Mus musculus
physiological function
tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production
Rattus norvegicus
Other publictions for EC 6.3.2.24
745971
Tsukahara
Tyrosyl-tRNA synthetase A pot ...
Homo sapiens, Mus musculus, Mus musculus C57BL/6, Rattus norvegicus
Peptides
101
60-68
2018
-
-
1
-
-
-
-
3
-
1
-
-
-
145
-
-
1
-
-
19
-
-
8
1
8
3
1
-
-
1
1
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
3
-
1
-
-
-
-
-
1
-
19
-
-
8
1
3
1
-
-
1
1
-
-
-
5
5
-
-
-
648948
Kawabata
Kyotorphin synthetase activity ...
Rattus norvegicus
Peptides
17
407-411
1996
-
-
-
-
-
-
4
5
1
1
1
-
-
2
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
5
1
1
1
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648949
Kawabata
NG-nitro-L-arginine methyl est ...
Rattus norvegicus
Peptides
16
1317-1319
1995
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648947
Ueda
Kyotorphin (tyrosine-arginine) ...
Rattus norvegicus
J. Biol. Chem.
262
8165-8173
1987
-
-
-
-
-
-
6
4
2
2
1
1
-
2
-
-
1
-
-
1
1
-
14
-
-
1
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
6
-
4
2
2
1
1
-
-
-
1
-
1
1
-
14
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-