BRENDA - Reference to 6.3.2.24; Id = 745971

expression in Escherichia coli; real-time PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)

Homo sapiens

additional information

-

additional information

Michaelis-Menten kinetics

Homo sapiens

0.2

-

L-tyrosine

pH 7.4, 37°C; pH 7.4, 37°C, recombinant His-tagged enzyme

Homo sapiens

1.4

-

L-arginine

pH 7.4, 37°C; pH 7.4, 37°C, recombinant His-tagged enzyme

Homo sapiens

Mg2+

required. Assay in presence of 100 mM

Homo sapiens

-

Mus musculus

-

Mus musculus C57BL/6

-

Rattus norvegicus

-

recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Homo sapiens

adrenal gland

-

Homo sapiens

-

amygdala

-

Mus musculus

-

brain

-

Rattus norvegicus

-

brain

-

Homo sapiens

-

brain

expression pattern in mouse brain of TyrRS and KTP synthesis after Arg administration

Mus musculus

-

cerebellum

-

Mus musculus

-

cerebral cortex

-

Mus musculus

-

hippocampus

-

Mus musculus

-

hypothalamus

-

Mus musculus

-

medulla oblongata

; TyrRS levels are greater in the midbrain and medulla oblongata than in other brain regions. When arginine is administered 2 h prior to brain dissection, the kyotorphin levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even

Mus musculus

-

midbrain

; TyrRS levels are greater in the midbrain and medulla oblongata than in other brain regions. When arginine is administered 2 h prior to brain dissection, the kyotorphin levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even

Mus musculus

-

additional information

expression in all organs tested

Homo sapiens

-

olfactory bulb

-

Mus musculus

-

pancreas

; highest expression level

Homo sapiens

-

PC-12 cell

-

Rattus norvegicus

-

skeletal muscle

-

Homo sapiens

-

striatum

-

Mus musculus

-

testis

-

Homo sapiens

-

thalamus

-

Mus musculus

-

ATP + L-tyrosine + L-arginine

-

745971

Mus musculus

AMP + diphosphate + L-tyrosyl-L-arginine

-

?

ATP + L-tyrosine + L-arginine

-

745971

Homo sapiens

AMP + diphosphate + L-tyrosyl-L-arginine

-

?

ATP + L-tyrosine + L-arginine

-

745971

Rattus norvegicus

AMP + diphosphate + L-tyrosyl-L-arginine

-

?

ATP + L-tyrosine + L-arginine

-

745971

Mus musculus C57BL/6

AMP + diphosphate + L-tyrosyl-L-arginine

-

?

additional information

recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1

745971

Mus musculus

?

-

additional information

recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1

745971

Homo sapiens

?

-

additional information

recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1

745971

Rattus norvegicus

?

-

additional information

recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1

745971

Mus musculus C57BL/6

?

-

?

x * 59000, recombinant His6-tagged enzyme, SDS-PAGE

Homo sapiens

KTP synthetase

-

Homo sapiens

KTP synthetase

-

Mus musculus

KTP synthetase

-

Rattus norvegicus

kyotorphin synthetase

-

Homo sapiens

kyotorphin synthetase

-

Mus musculus

kyotorphin synthetase

-

Rattus norvegicus

TyrRS

-

Homo sapiens

TyrRS

-

Mus musculus

37

-

assay at

Mus musculus

37

-

assay at

Rattus norvegicus

50

-

Homo sapiens

37

70

60% of maximal activity at 37°C , 30% at 70°C , maximal activity at 50°C, no activity at 100°C

Homo sapiens

7.4

-

Homo sapiens

2.2

9.5

KTP production is highest at pH 7.4, and is approximately half of that at pH 9.5. Production is markedly lower under acidic conditions at pH 3.8 and pH 2.2

Homo sapiens

ATP

-

Homo sapiens

ATP

-

Mus musculus

ATP

-

Rattus norvegicus

expression in Escherichia coli; real-time PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)

Homo sapiens

ATP

-

Homo sapiens

ATP

-

Mus musculus

ATP

-

Rattus norvegicus

additional information

-

additional information

Michaelis-Menten kinetics

Homo sapiens

0.2

-

L-tyrosine

pH 7.4, 37°C; pH 7.4, 37°C, recombinant His-tagged enzyme

Homo sapiens

1.4

-

L-arginine

pH 7.4, 37°C; pH 7.4, 37°C, recombinant His-tagged enzyme

Homo sapiens

Mg2+

required. Assay in presence of 100 mM

Homo sapiens

recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Homo sapiens

adrenal gland

-

Homo sapiens

-

amygdala

-

Mus musculus

-

brain

-

Rattus norvegicus

-

brain

-

Homo sapiens

-

brain

expression pattern in mouse brain of TyrRS and KTP synthesis after Arg administration

Mus musculus

-

cerebellum

-

Mus musculus

-

cerebral cortex

-

Mus musculus

-

hippocampus

-

Mus musculus

-

hypothalamus

-

Mus musculus

-

medulla oblongata

; TyrRS levels are greater in the midbrain and medulla oblongata than in other brain regions. When arginine is administered 2 h prior to brain dissection, the kyotorphin levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even

Mus musculus

-

midbrain

; TyrRS levels are greater in the midbrain and medulla oblongata than in other brain regions. When arginine is administered 2 h prior to brain dissection, the kyotorphin levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even

Mus musculus

-

additional information

expression in all organs tested

Homo sapiens

-

olfactory bulb

-

Mus musculus

-

pancreas

; highest expression level

Homo sapiens

-

PC-12 cell

-

Rattus norvegicus

-

skeletal muscle

-

Homo sapiens

-

striatum

-

Mus musculus

-

testis

-

Homo sapiens

-

thalamus

-

Mus musculus

-

ATP + L-tyrosine + L-arginine

-

745971

Mus musculus

AMP + diphosphate + L-tyrosyl-L-arginine

-

?

ATP + L-tyrosine + L-arginine

-

745971

Homo sapiens

AMP + diphosphate + L-tyrosyl-L-arginine

-

?

ATP + L-tyrosine + L-arginine

-

745971

Rattus norvegicus

AMP + diphosphate + L-tyrosyl-L-arginine

-

?

ATP + L-tyrosine + L-arginine

-

745971

Mus musculus C57BL/6

AMP + diphosphate + L-tyrosyl-L-arginine

-

?

additional information

recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1

745971

Mus musculus

?

-

additional information

recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1

745971

Homo sapiens

?

-

additional information

recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1

745971

Rattus norvegicus

?

-

additional information

recombinant hTyrRS also shows tyrosine tRNA ligase activity, cf. EC 6.1.1.1

745971

Mus musculus C57BL/6

?

-

?

x * 59000, recombinant His6-tagged enzyme, SDS-PAGE

Homo sapiens

37

-

assay at

Mus musculus

37

-

assay at

Rattus norvegicus

50

-

Homo sapiens

37

70

60% of maximal activity at 37°C , 30% at 70°C , maximal activity at 50°C, no activity at 100°C

Homo sapiens

7.4

-

Homo sapiens

2.2

9.5

KTP production is highest at pH 7.4, and is approximately half of that at pH 9.5. Production is markedly lower under acidic conditions at pH 3.8 and pH 2.2

Homo sapiens

malfunction

when arginine is administered 2 h prior to brain dissection, the KTP levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even

Mus musculus

malfunction

TyrRS knockdown of PC12 cells with a small interfering RNA (siRNA) in the presence of 1.6mM tyrosine, arginine, proline, or tryptophan significantly reduces the level of KTP, but not those of tyrosine-tyrosine, tyrosine-proline, or tyrosine-tryptophan. siRNA treatment does not affect cell survival or proliferation

Rattus norvegicus

physiological function

tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production; TyrRS knockdown of PC12 cells with siRNA in the presence of 1.6 mM tyrosine, arginine, proline, or tryptophan significantly reduces the level of product kyotorphin, but not those of tyrosine-tyrosine, tyrosine-proline, or tyrosine-tryptophan. siRNA treatment does not affect cell survival or proliferation

Homo sapiens

physiological function

tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production

Mus musculus

physiological function

tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production

Rattus norvegicus

malfunction

when arginine is administered 2 h prior to brain dissection, the KTP levels in these regions plus olfactory bulb significantly increase, although basal brain KTP levels remain relatively even

Mus musculus

malfunction

TyrRS knockdown of PC12 cells with a small interfering RNA (siRNA) in the presence of 1.6mM tyrosine, arginine, proline, or tryptophan significantly reduces the level of KTP, but not those of tyrosine-tyrosine, tyrosine-proline, or tyrosine-tryptophan. siRNA treatment does not affect cell survival or proliferation

Rattus norvegicus

physiological function

tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production; TyrRS knockdown of PC12 cells with siRNA in the presence of 1.6 mM tyrosine, arginine, proline, or tryptophan significantly reduces the level of product kyotorphin, but not those of tyrosine-tyrosine, tyrosine-proline, or tyrosine-tryptophan. siRNA treatment does not affect cell survival or proliferation

Homo sapiens

physiological function

tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production

Mus musculus

physiological function

tyrosyl-tRNA synthetase is a potential kyotorphin synthetase (i.e. tyrosine-arginine ligase, EC 6.3.2.24) in mammals, positive correlation across brain regions between TyrRS expression and arginine-accelerated KTP production

Rattus norvegicus

745971

Tsukahara

Tyrosyl-tRNA synthetase A pot ...

Homo sapiens, Mus musculus, Mus musculus C57BL/6, Rattus norvegicus

Peptides

101

60-68

2018

-

1

-

3

-

1

-

145

-

1

-

19

-

8

1

8

3

1

-

1

-

3

-

1

3

-

3

-

1

-

1

-

19

-

8

1

3

1

-

1

-

5

-

648948

Kawabata

Kyotorphin synthetase activity ...

Rattus norvegicus

Peptides

17

407-411

1996

-

4

5

1

-

2

-

4

-

1

-

4

-

5

1

-

4

-

1

-

648949

Kawabata

NG-nitro-L-arginine methyl est ...

Rattus norvegicus

Peptides

16

1317-1319

1995

-

2

-

1

-

2

-

2

-

1

-

2

-

1

-

2

-

1

-

648947

Ueda

Kyotorphin (tyrosine-arginine) ...

Rattus norvegicus

J. Biol. Chem.

262

8165-8173

1987

-

6

4

2

1

-

2

-

1

-

1

-

14

-

1

-

1

-

1

-

6

-

4

2

1

-

1

-

1

-

14

-

1

-

1

-

1

-

Tyrosyl-tRNA synthetase A potential kyotorphin synthetase in mammals

Data extracted from this reference: