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Literature summary for 6.3.2.23 extracted from
- Thiol synthetases of legumes: immunogold localization and differential gene regulation by phytohormones (2012), J. Exp. Bot., 63, 3923-3934.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| auxin | activates enzyme activity | Phaseolus vulgaris |  |
| auxin | activates enzyme activity | Medicago sativa | |
| auxin | activates enzyme activity | Lotus japonicus | |
| additional information | no activation by cyctokinins and polyamines | Phaseolus vulgaris | |
| additional information | no activation by cyctokinins and polyamines | Medicago sativa | |
| additional information | no activation by cyctokinins and polyamines | Lotus japonicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of N-terminally maltose binding protein- and His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Phaseolus vulgaris |
| recombinant expression of N-terminally maltose binding protein- and His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Medicago sativa |
| recombinant expression of N-terminally maltose binding protein- and His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Lotus japonicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Phaseolus vulgaris | 9507 | - |
| chloroplast | - |
Medicago sativa | 9507 | - |
| chloroplast | - |
Lotus japonicus | 9507 | - |
| cytosol | - |
Phaseolus vulgaris | 5829 | - |
| cytosol | - |
Medicago sativa | 5829 | - |
| cytosol | - |
Lotus japonicus | 5829 | - |
| additional information | immunolocalization studies of thiol synthetases in leaves, roots, and nodules, overview | Medicago sativa | - |
- |
| additional information | immunolocalization studies of thiol synthetases in leaves, roots, and nodules, overview | Lotus japonicus | - |
- |
| plastid | - |
Phaseolus vulgaris | 9536 | - |
| plastid | - |
Medicago sativa | 9536 | - |
| plastid | - |
Lotus japonicus | 9536 | - |
| starch grain | of leaf chloroplasts and root plastids | Medicago sativa | 43036 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Phaseolus vulgaris | |
| Mg2+ | required | Medicago sativa | |
| Mg2+ | required | Lotus japonicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, about, recombinant tagged enzyme, SDS-PAGE | Phaseolus vulgaris |
| 50000 | - |
x * 50000, about, recombinant tagged enzyme, SDS-PAGE | Medicago sativa |
| 50000 | - |
x * 50000, about, recombinant tagged enzyme, SDS-PAGE | Lotus japonicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-L-glutamyl-L-cysteine + beta-alanine | Phaseolus vulgaris | - |
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine | i.e. homo-glutathione | ? | |
| ATP + gamma-L-glutamyl-L-cysteine + beta-alanine | Medicago sativa | - |
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine | i.e. homo-glutathione | ? | |
| ATP + gamma-L-glutamyl-L-cysteine + beta-alanine | Lotus japonicus | - |
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine | i.e. homo-glutathione | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lotus japonicus | - |
ecotype MG-20, in nodulated plants x Mesorhizobium loti R7A | - |
| Medicago sativa | - |
cv. Aragon x Sinorhizobium meliloti 102F78 | - |
| Phaseolus vulgaris | - |
cv. Contender x Rhizobium leguminosarum cv. phaseoli 3622 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally maltose binding protein- and His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and immuno affinity chromatography | Phaseolus vulgaris |
| recombinant N-terminally maltose binding protein- and His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and immuno affinity chromatography | Medicago sativa |
| recombinant N-terminally maltose binding protein- and His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and immuno affinity chromatography | Lotus japonicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Lotus japonicus | - |
| leaf | mainly in starch grains within chloroplasts | Medicago sativa | - |
| additional information | immunolocalization studies of thiol synthetases in leaves, roots, and nodules, overview | Phaseolus vulgaris | - |
| root | nodulated plants | Phaseolus vulgaris | - |
| root | nodulated plants, mainly in starch grains within plastids | Medicago sativa | - |
| root | nonnodulated and nodulated plants | Lotus japonicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-L-glutamyl-L-cysteine + beta-alanine | - |
Phaseolus vulgaris | ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine | i.e. homo-glutathione | ? | |
| ATP + gamma-L-glutamyl-L-cysteine + beta-alanine | - |
Medicago sativa | ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine | i.e. homo-glutathione | ? | |
| ATP + gamma-L-glutamyl-L-cysteine + beta-alanine | - |
Lotus japonicus | ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine | i.e. homo-glutathione | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, about, recombinant tagged enzyme, SDS-PAGE | Phaseolus vulgaris |
| ? | x * 50000, about, recombinant tagged enzyme, SDS-PAGE | Medicago sativa |
| ? | x * 50000, about, recombinant tagged enzyme, SDS-PAGE | Lotus japonicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hGSHS | - |
Phaseolus vulgaris |
| hGSHS | - |
Medicago sativa |
| hGSHS | - |
Lotus japonicus |
| homoglutathione synthetase | - |
Phaseolus vulgaris |
| homoglutathione synthetase | - |
Medicago sativa |
| homoglutathione synthetase | - |
Lotus japonicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Phaseolus vulgaris |
| 30 | - |
assay at | Medicago sativa |
| 30 | - |
assay at | Lotus japonicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Phaseolus vulgaris |
| 8.5 | - |
assay at | Medicago sativa |
| 8.5 | - |
assay at | Lotus japonicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Phaseolus vulgaris | |
| ATP | - |
Medicago sativa | |
| ATP | - |
Lotus japonicus | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Lotus japonicus | abscisic acid downregulates the enzyme in nonnodulated plants, salicylic acid downregulates the enzyme slightly in nodulated plants, jasmonic acid, and auxin downregulate the enzyme in roots, cytokinins and polyamines reduce the enzyme slightly in nodulated plants | down |
| Lotus japonicus | abscisic acid upregulates the enzyme slightly in nodulated plants, salicylic acid upregulates the enzyme slightly in nonnodulated plants, indole-3-acetic acid induces the enzyme slightly in nodulated plants | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the response profile of GSH and hGSH in nonnodulated and nodulated plants to treatment with various hormones indicate that thiol homeostasis is independent of the nitrogen source of the plants | Lotus japonicus |
| physiological function | differential regulation of mRNA levels, enzyme activity, and thiol contents by hormones, overview. Glutathione and homoglutathione play distinct roles in plant development and stress response | Lotus japonicus |
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