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Literature summary for 6.3.2.2 extracted from

  • Song, W.; Yuan, J.; Zhang, Z.; Li, L.; Hu, L.
    Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus (2014), Exp. Ther. Med., 8, 195-200 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.2

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + L-cysteine Homo sapiens
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P48506 AND P48507 genes GCLC and GCLM encoding catalytic and modifier subunits
-

Source Tissue

Source Tissue Comment Organism Textmining
peripheral blood mononuclear cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + L-cysteine
-
 Homo sapiens ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
heterodimer GCL is a heterodimeric enzyme, consisting of a catalytic subunit, (GCLc) and a modulatory subunit (GCLm) that are encoded by two distinct genes. GCLc constitutes all the enzymatic activity, but catalytic efficiency is increased substantially by covalent interaction with GCLm Homo sapiens

Synonyms

Synonyms Comment Organism
GCL
-
 Homo sapiens
glutamate cysteine ligase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction in patients with systemic lupus erythematosus (SLE), the levels of enzyme GCL activity and reduced glutathione (GSH) decrease, while thioredoxin (TRX) and oxidized glutathione (GSSG) levels increase when compared with those in the healthy controls. GSH concentrations and GCL activity levels negatively correlate with the SLE disease activity index and erythrocyte sedimentation rate. Patients with SLE and nephritis have lower levels of GSH and GCL activity and higher levels of TRX and GSSG compared with those in SLE patients without nephritis. Insufficient levels of GSH and GCL activity in PBMCs may contribute to the pathogenesis of SLE. A negative association of GSH levels with T-lymphocyte and CD4+ and CD8+ lymphocyte subset apoptosis, and intracellular activated caspase?3 may supports the role of GSH in the altera­tion of apoptosis of T lymphocytes in the SLE disease state. GSH is involved in the depletion of CD4+ T lymphocytes in patients with SLE Homo sapiens
metabolism glutamate cysteine ligase (GCL) catalyzes the first and rate-limiting step of glutathione, GSH, biosynthesis. The associations between GSH levels and GCL activity with demographic character­istics, clinical manifestations and laboratory parameters in peripheral blood mononuclear cells (PBMCs) are analyzed, overview Homo sapiens
additional information GCL is a heterodimeric enzyme, consisting of a catalytic subunit, (GCLc) and a modulatory subunit (GCLm) that are encoded by two distinct genes. GCLc constitutes all the enzymatic activity, but catalytic efficiency is increased substantially by covalent interaction with GCLm Homo sapiens