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Literature summary for 6.3.2.2 extracted from

  • Li, M.; Zhang, Z.; Yuan, J.; Zhang, Y.; Jin, X.
    Altered glutamate cysteine ligase expression and activity in renal cell carcinoma (2014), Biomed. Rep., 2, 831-834 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.2

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + L-cysteine Homo sapiens
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P48506 AND P48507 genes GCLC and GCLM encoding heavy and light chain subunits
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Homo sapiens
-
renal cancer cell the protein expression levels of the GCL subunits (catalytic subunit, GCLc, and modulatory subunit, GCLm) and GCL activity are both significantly increased in renal cancer tissue Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + L-cysteine
-
 Homo sapiens ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
heterodimer enzyme GCL is a heterodi­meric enzyme consisting of a catalytic subunit (GCLc) and a modulatory subunit (GCLm), which are encoded by two genes. The catalytic subunit GCLc performs all the enzymatic activity and its catalytic efficiency is increased by the covalent interaction with the regulatory subunit GCLm Homo sapiens

Synonyms

Synonyms Comment Organism
gamma-Glutamylcysteine synthetase
-
 Homo sapiens
GCL
-
 Homo sapiens
GCLC
-
 Homo sapiens
GCLM
-
 Homo sapiens
glutamate cysteine ligase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens specific downregulation of the GCL levels by hammerhead ribo­zyme down

General Information

General Information Comment Organism
malfunction GSH can be depleted through the specific downregulation of the GCL levels by hammerhead ribo­zyme Homo sapiens
physiological function GSH is synthesized de novo in a two-step process catalyzed by glutamate cysteine ligase (GCL, EC 6.3.2.2), and glutathione synthetase (GS, EC 6.3.2.3). GCL catalyzes the first and rate-limiting step, in which glutamate is ligated with cysteine to form gamma-glutamylcysteine (gamma-GC), which is rapidly linked to glycine to form GSH via the action of glutathione synthetase. Increased expression and enzymatic activity of enzyme GCL is closely associated with renal cell carcinoma (RCC) suggesting an important role for glutathione in the pathogenesis of RCC Homo sapiens