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Literature summary for 6.3.2.2 extracted from

  • Wu, H.; White, C.C.; Isanhart, J.P.; McBride, T.J.; Kavanagh, T.J.; Hooper, M.J.
    Optimization and application of glutamate cysteine ligase measurement in wildlife species (2008), Ecotoxicol. Environ. Saf., 72, 572-578.
    View publication on PubMed
Search for more literature for 6.3.2.2

Activating Compound

Activating Compound Comment Organism Structure
L-cysteine varying glutamic acid concentrations from 5 to 80 mM do not affect GCL activities markedly, whereas cysteine concentrations from 2.5 to 40 mM influence GCL activities substantially in a tissue-dependent manner, about 20 mM L-Cys is optimal in the different tissue, overview. After subacute exposure, low doses increases GCL activity and GSH content in liver by 48.3% and 54.4%, respectively. High doses reduce GCL activities significantly in liver and kidney to 31.2% and 43.0% of the control, respectively Anas platyrhynchos
L-cysteine varying glutamic acid concentrations from 5 to 80 mM do not affect GCL activities markedly, whereas cysteine concentrations from 2.5 to 40 mM influence GCL activities substantially in a tissue-dependent manner, about 20 mM L-Cys is optimal in the different tissue, overview. Low doses activate high doses inhibits the enzyme Mus musculus
L-cysteine varying glutamic acid concentrations from 5 to 80 mM do not affect GCL activities markedly, whereas cysteine concentrations from 2.5 to 40 mM influence GCL activities substantially in a tissue-dependent manner, about 20 mM L-Cys is optimal in the different tissue, overview. Low doses activate high doses inhibits the enzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
L-cysteine varying glutamic acid concentrations from 5 to 80 mM do not affect GCL activities markedly, whereas cysteine concentrations from 2.5 to 40 mM influence GCL activities substantially in a tissue-dependent manner, about 20 mM L-Cys is optimal in the different tissue, overview. After subacute exposure, low doses increases GCL activity and GSH content in liver by 48.3% and 54.4%, respectively. High doses reduce GCL activities significantly in liver and kidney to 31.2% and 43.0% of the control, respectively Anas platyrhynchos
L-cysteine varying glutamic acid concentrations from 5 to 80 mM do not affect GCL activities markedly, whereas cysteine concentrations from 2.5 to 40 mM influence GCL activities substantially in a tissue-dependent manner, about 20 mM L-Cys is optimal in the different tissue, overview. Low doses activate high doses inhibits the enzyme Mus musculus
L-cysteine varying glutamic acid concentrations from 5 to 80 mM do not affect GCL activities markedly, whereas cysteine concentrations from 2.5 to 40 mM influence GCL activities substantially in a tissue-dependent manner, about 20 mM L-Cys is optimal in the different tissue, overview. Low doses activate high doses inhibits the enzyme Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mus musculus
Mg2+
-
 Rattus norvegicus
Mg2+
-
 Anas platyrhynchos

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + L-cysteine Mus musculus rate-limiting enzyme in glutathione biosynthesis ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
ATP + L-glutamate + L-cysteine Rattus norvegicus rate-limiting enzyme in glutathione biosynthesis ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
ATP + L-glutamate + L-cysteine Anas platyrhynchos rate-limiting enzyme in glutathione biosynthesis ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Anas platyrhynchos
-
 mallard duck
-
Mus musculus
-
 deer mice
-
Rattus norvegicus
-
 Sprague-Dawley rats
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-
brain
-
 Rattus norvegicus
-
brain
-
 Anas platyrhynchos
-
heart
-
 Mus musculus
-
heart
-
 Rattus norvegicus
-
heart
-
 Anas platyrhynchos
-
kidney
-
 Mus musculus
-
kidney
-
 Rattus norvegicus
-
kidney
-
 Anas platyrhynchos
-
liver
-
 Mus musculus
-
liver
-
 Rattus norvegicus
-
liver
-
 Anas platyrhynchos
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mus musculus
additional information
-
-
 Rattus norvegicus
additional information
-
-
 Anas platyrhynchos

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + L-cysteine
-
 Mus musculus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
ATP + L-glutamate + L-cysteine
-
 Rattus norvegicus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
ATP + L-glutamate + L-cysteine
-
 Anas platyrhynchos ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
ATP + L-glutamate + L-cysteine rate-limiting enzyme in glutathione biosynthesis Mus musculus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
ATP + L-glutamate + L-cysteine rate-limiting enzyme in glutathione biosynthesis Rattus norvegicus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
ATP + L-glutamate + L-cysteine rate-limiting enzyme in glutathione biosynthesis Anas platyrhynchos ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

Synonyms

Synonyms Comment Organism
GCL
-
 Mus musculus
GCL
-
 Rattus norvegicus
GCL
-
 Anas platyrhynchos
glutamate cysteine ligase
-
 Mus musculus
glutamate cysteine ligase
-
 Rattus norvegicus
glutamate cysteine ligase
-
 Anas platyrhynchos

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus
ATP
-
 Rattus norvegicus
ATP
-
 Anas platyrhynchos