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Literature summary for 6.3.2.2 extracted from

  • Toroser, D.; Yarian, C.S.; Orr, W.C.; Sohal, R.S.
    Mechanisms of gamma-glutamylcysteine ligase regulation (2006), Biochim. Biophys. Acta, 1760, 233-244.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.2

Inhibitors

Inhibitors Comment Organism Structure
buthionine sulfoximine
-
 Drosophila melanogaster
MgATP2- although the enzyme preparation shows a strict requirement for MgATP2- for gamma-glutamylcysteine synthesis, preincubation of the homogenate under phosphorylating conditions with MgATP2- also causes a maximal inhibition of 89% Drosophila melanogaster
MgATP2- although the enzyme preparation shows a strict requirement for MgATP2- for gamma-glutamylcysteine synthesis, preincubation of the homogenates under phosphorylating conditions with MgATP2- also causes a maximal inhibition of 94%, 77%, 85%, 87%, 83% and 95% in cerebellum, hippocampus, brainstem, striatum, cortex and heart Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mus musculus
Mg2+ required Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + L-cysteine Mus musculus the mechanism of modulation of eukaryotic gamma-glutamylcysteine ligase enzymes may include specific binding of ligands such as pyridine dinucleotide phosphates and reversible protein phosphorylation ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9W3K5
-
-
Mus musculus P97494
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the enzyme exists in vivo as a mixture of phosphorylated and dephosphorylated forms Mus musculus
phosphoprotein the enzyme exists in vivo as a mixture of phosphorylated and dephosphorylated forms Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
brain stem
-
 Mus musculus
-
cerebellum
-
 Mus musculus
-
heart
-
 Mus musculus
-
hippocampus
-
 Mus musculus
-
neostriatum
-
 Mus musculus
-
temporal lobe
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + L-cysteine
-
 Mus musculus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
ATP + L-glutamate + L-cysteine
-
 Drosophila melanogaster ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
ATP + L-glutamate + L-cysteine the mechanism of modulation of eukaryotic gamma-glutamylcysteine ligase enzymes may include specific binding of ligands such as pyridine dinucleotide phosphates and reversible protein phosphorylation Mus musculus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 in presence of NADPH Drosophila melanogaster
8.2
-
 in absence of NADPH Drosophila melanogaster