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Literature summary for 6.3.2.2 extracted from

  • Chen, Y.; Shertzer, H.G.; Schneider, S.N.; Nebert, D.W.; Dalton, T.P.
    Glutamate cysteine ligase catalysis: dependence on ATP and modifier subunit for regulation of tissue glutathione levels (2005), J. Biol. Chem., 280, 33766-33774.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
GSH noncompetitive versus L-glutamate or ATP Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
L-cysteine 37°C, pH 7.2, holoenzyme Mus musculus
0.27
-
L-cysteine 37°C, pH 7.2, catalytic subunit GCLC Mus musculus
0.48
-
L-glutamate 37°C, pH 7.2, holoenzyme Mus musculus
0.87
-
ATP 37°C, pH 7.2, holoenzyme Mus musculus
1.6
-
L-glutamate 37°C, pH 7.2, catalytic subunit GCLC Mus musculus
5
-
ATP 37°C, pH 7.2, catalytic subunit GCLC Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus rate-limiting enzyme in GSH biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Mus musculus
-
liver
-
Mus musculus
-
lung
-
Mus musculus
-
additional information in most tissues the modifier subunit GCLM is limiting, suggesting that an increase in GCLM alone would increase gamma-glutamylcysteine synthesis Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + L-cysteine
-
Mus musculus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
?
additional information rate-limiting enzyme in GSH biosynthesis Mus musculus ?
-
?

Subunits

Subunits Comment Organism
More reaction is catalyzed by the catalytic subunit GCLC or by the holoenzyme (GCLholo), which comprises GCLC and the modifier subunit GCLM. GCLM decreases the Km for ATP by about 6fold and decreases the Km-value for glutamate and increases the Ki-value for feedback inhibition by GSH. GCLM increases by 4.4fold the turnover number for gamma-glutamylcysteine synthesis Mus musculus

Synonyms

Synonyms Comment Organism
GCL
-
Mus musculus
glutamate cysteine ligase
-
Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.3
-
GSH Kis, 37°C, pH 7.2, catalytic subunit GCLC, versus L-glutamate Mus musculus
0.4
-
GSH Kii, 37°C, pH 7.2, catalytic subunit GCLC, versus ATP Mus musculus
0.8
-
GSH Kii, 37°C, pH 7.2, catalytic subunit GCLC, versus L-glutamate Mus musculus
0.8
-
GSH Kis, 37°C, pH 7.2, holoenzyme, versus L-glutamate Mus musculus
1.3
-
GSH Kis, 37°C, pH 7.2, catalytic subunit GCLC, versus ATP Mus musculus
3.1
-
GSH Kii, 37°C, pH 7.2, holoenzyme, versus L-glutamate Mus musculus
3.9
-
GSH Kii, 37°C, pH 7.2, holoenzyme, versus ATP Mus musculus
6.5
-
GSH Kis, 37°C, pH 7.2, holoenzyme, versus ATP Mus musculus