Literature summary for 6.3.2.2 extracted from

Huang, C.S.; Moore, W.R.; Meister, A.
On the active site thiol of gamma-glutamylcysteine synthetase: relationship to catalysis, inhibition, and regulation (1988), Proc. Natl. Acad. Sci. USA, 85, 2464-2468.

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Inhibitors

Inhibitors	Comment	Organism	Structure
4-Methylene glutamate	no inhibition	Escherichia coli
4-Methylene glutamate	-	Rattus norvegicus
buthionine sulfoximine	only in presence of ATP	Escherichia coli
buthionine sulfoximine	-	Rattus norvegicus
cystamine	no inhibition	Escherichia coli
cystamine	-	Rattus norvegicus
glutathione	feed-back inhibition	Escherichia coli
glutathione	feed-back inhibition	Rattus norvegicus
S-sulfocysteine	no inhibition	Escherichia coli
S-sulfocysteine	-	Rattus norvegicus
S-sulfohomocysteine	no inhibition	Escherichia coli
S-sulfohomocysteine	-	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	L-Cys	strain W	Escherichia coli
0.2	-	L-Cys	-	Rattus norvegicus
0.2	-	L-Cys	strain KM	Escherichia coli
0.7	-	L-Glu	strain W	Escherichia coli
1	-	L-2-aminobutanoate	-	Rattus norvegicus
1.3	-	L-2-aminobutanoate	-	Rattus norvegicus
1.3	-	L-2-aminobutanoate	strain W	Escherichia coli
1.4	-	L-2-aminobutanoate	-	Rattus norvegicus
1.4	-	L-2-aminobutanoate	strain KM	Escherichia coli
1.7	-	L-Glu	strain KM	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56000	-	gel filtration, native PAGE	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	enriched by recombinant DNA techniques in its content of gamma-glutamylcysteine synthetase, strain W, strain KM	-
Rattus norvegicus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
31	-	-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-Glu + beta-chloro-L-Ala	-	Rattus norvegicus	ADP + phosphate + gamma-L-Glu-L-beta-chloro-L-Ala	-	?
ATP + L-Glu + beta-chloro-L-Ala	strain KM: 79% of the activity relative to L-Cys, strain W: 99% of the activity relative to L-Cys	Escherichia coli	ADP + phosphate + gamma-L-Glu-L-beta-chloro-L-Ala	-	?
ATP + L-Glu + L-2-aminobutanoate	-	Rattus norvegicus	ADP + phosphate + gamma-L-Glu-2-aminobutanoate	-	?
ATP + L-Glu + L-2-aminobutanoate	strain KM: 85% of the activity relative to L-Cys, strain W: 81% of the activity relative to L-Cys	Escherichia coli	ADP + phosphate + gamma-L-Glu-2-aminobutanoate	-	?
ATP + L-Glu + L-Cys	-	Escherichia coli	ADP + phosphate + gamma-L-Glu-L-Cys	-	?
ATP + L-Glu + L-Cys	-	Rattus norvegicus	ADP + phosphate + gamma-L-Glu-L-Cys	-	?
ATP + L-Glu + L-Ser	strain KM: 18% of the activity relative to L-Cys, strain W: 13% of the activity relative to L-Cys	Escherichia coli	ADP + phosphate + gamma-L-Glu-L-Ser	-	?
ATP + L-Glu + S-methyl-L-Cys	-	Rattus norvegicus	ADP + phosphate + gamma-L-Glu-S-methyl-L-Cys	-	?
ATP + L-Glu + S-methyl-L-Cys	strain KM: 70% of the activity relative to L-Cys, strain W: 70% of the activity relative to L-Cys	Escherichia coli	ADP + phosphate + gamma-L-Glu-S-methyl-L-Cys	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 56000, SDS-PAGE	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Escherichia coli
additional information	-	additional information	-	Rattus norvegicus

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Release 2023.1 (January 2023)