BRENDA - Enzyme Database show
show all sequences of 6.3.2.16

D-Alanine:membrane acceptor ligase from Lactobacillus casei

Reusch, V.M.; Neuhaus, F.C.; J. Biol. Chem. 246, 6136-6143 (1971)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Lactobacillus casei
D-2-amino-n-butanoate
-
Lactobacillus casei
D-Ser
-
Lactobacillus casei
DL-2-Amino-n-butanoyl hydroxamate
-
Lactobacillus casei
DL-Ala amide
-
Lactobacillus casei
DL-Alanine hydroxamate
-
Lactobacillus casei
Ni2+
-
Lactobacillus casei
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
D-Ala
-
Lactobacillus casei
0.85
-
D-2-amino-n-butanoate
-
Lactobacillus casei
3.3
-
ATP
-
Lactobacillus casei
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
stimulates
Lactobacillus casei
Ca2+
stimulates
Lactobacillus casei
Co2+
stimulates
Lactobacillus casei
Mg2+
stimulates
Lactobacillus casei
Mn2+
stimulates
Lactobacillus casei
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
gel filtration
Lactobacillus casei
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + D-Ala + membrane-acceptor
Lactobacillus casei
reaction is responsible for the incorporation of D-Ala into membranes of Lactobacillus casei, involved in the synthesis of teichoic acids
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactobacillus casei
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + D-Ala + membrane-acceptor
83% of the activity relative to ATP
1056
Lactobacillus casei
AMP + phosphate + D-alanyl-membrane acceptor
-
-
-
-
ATP + D-2-amino-n-butanoate + membrane-acceptor
-
1056
Lactobacillus casei
ADP + phosphate + D-2-aminobutanoyl-membrane acceptor
-
-
-
-
ATP + D-Ala + membrane-acceptor
no activity with L-Ala
1056
Lactobacillus casei
ADP + phosphate + D-alanyl-membrane acceptor
-
1056
Lactobacillus casei
-
ATP + D-Ala + membrane-acceptor
reaction is responsible for the incorporation of D-Ala into membranes of Lactobacillus casei, involved in the synthesis of teichoic acids
1056
Lactobacillus casei
?
-
-
-
-
CTP + D-Ala + membrane-acceptor
107% of the activity relative to ATP
1056
Lactobacillus casei
CDP + phosphate + D-alanyl-membrane acceptor
-
-
-
-
GTP + D-Ala + membrane-acceptor
84% of the activity relative to ATP
1056
Lactobacillus casei
GDP + phosphate + D-alanyl-membrane acceptor
-
-
-
-
UTP + D-Ala + membrane-membrane acceptor
126% of the activity relative to ATP
1056
Lactobacillus casei
UDP + phosphate + D-alanyl-membrane acceptor
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
-
Lactobacillus casei
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Lactobacillus casei
D-2-amino-n-butanoate
-
Lactobacillus casei
D-Ser
-
Lactobacillus casei
DL-2-Amino-n-butanoyl hydroxamate
-
Lactobacillus casei
DL-Ala amide
-
Lactobacillus casei
DL-Alanine hydroxamate
-
Lactobacillus casei
Ni2+
-
Lactobacillus casei
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
D-Ala
-
Lactobacillus casei
0.85
-
D-2-amino-n-butanoate
-
Lactobacillus casei
3.3
-
ATP
-
Lactobacillus casei
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
stimulates
Lactobacillus casei
Ca2+
stimulates
Lactobacillus casei
Co2+
stimulates
Lactobacillus casei
Mg2+
stimulates
Lactobacillus casei
Mn2+
stimulates
Lactobacillus casei
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
gel filtration
Lactobacillus casei
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + D-Ala + membrane-acceptor
Lactobacillus casei
reaction is responsible for the incorporation of D-Ala into membranes of Lactobacillus casei, involved in the synthesis of teichoic acids
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + D-Ala + membrane-acceptor
83% of the activity relative to ATP
1056
Lactobacillus casei
AMP + phosphate + D-alanyl-membrane acceptor
-
-
-
-
ATP + D-2-amino-n-butanoate + membrane-acceptor
-
1056
Lactobacillus casei
ADP + phosphate + D-2-aminobutanoyl-membrane acceptor
-
-
-
-
ATP + D-Ala + membrane-acceptor
no activity with L-Ala
1056
Lactobacillus casei
ADP + phosphate + D-alanyl-membrane acceptor
-
1056
Lactobacillus casei
-
ATP + D-Ala + membrane-acceptor
reaction is responsible for the incorporation of D-Ala into membranes of Lactobacillus casei, involved in the synthesis of teichoic acids
1056
Lactobacillus casei
?
-
-
-
-
CTP + D-Ala + membrane-acceptor
107% of the activity relative to ATP
1056
Lactobacillus casei
CDP + phosphate + D-alanyl-membrane acceptor
-
-
-
-
GTP + D-Ala + membrane-acceptor
84% of the activity relative to ATP
1056
Lactobacillus casei
GDP + phosphate + D-alanyl-membrane acceptor
-
-
-
-
UTP + D-Ala + membrane-membrane acceptor
126% of the activity relative to ATP
1056
Lactobacillus casei
UDP + phosphate + D-alanyl-membrane acceptor
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
-
Lactobacillus casei
Other publictions for EC 6.3.2.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
1056
Reusch
D-Alanine:membrane acceptor li ...
Lactobacillus casei
J. Biol. Chem.
246
6136-6143
1971
-
-
-
-
-
-
7
3
-
5
1
1
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
3
-
5
1
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-