Cloned (Comment) | Organism |
---|---|
gene murE, recombinant overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)/pLysS, subcloning in Escherichia coli strain DH5alpha | Erysipelothrix rhusiopathiae |
Protein Variants | Comment | Organism |
---|---|---|
D399A | site-directed mutagenesis, inactive mutant | Erysipelothrix rhusiopathiae |
D399N | site-directed mutagenesis, almost inactive mutant | Erysipelothrix rhusiopathiae |
F340A | site-directed mutagenesis, almost inactive mutant | Erysipelothrix rhusiopathiae |
F340Y | site-directed mutagenesis, the mutant shows reduced activity with substrate L-alanine and meso-2,6-diaminoheptanedioate compared to wild-type enzyme | Erysipelothrix rhusiopathiae |
H398A | site-directed mutagenesis, the mutant shows reduced activity with substrate L-alanine and meso-2,6-diaminoheptanedioate compared to wild-type enzyme | Erysipelothrix rhusiopathiae |
H398D | site-directed mutagenesis, the mutant shows highly reduced activity with substrate L-alanine and meso-2,6-diaminoheptanedioate compared to wild-type enzyme | Erysipelothrix rhusiopathiae |
additional information | construction of an enzyme mutant MurEEr(HDNR/DNPR) with exchange of the HDNR morif to a DNPR motif, which is characteristic of meso-diaminopimelate-adding enzymes. The mutant shows increased activity with substrate meso-2,6-diaminoheptanedioate and reduced activity with substrate L-alanine | Erysipelothrix rhusiopathiae |
N400A | site-directed mutagenesis, the mutant shows reduced activity with substrate L-alanine and meso-2,6-diaminoheptanedioate compared to wild-type enzyme | Erysipelothrix rhusiopathiae |
N400P | site-directed mutagenesis, the mutant shows slightly reduced activity with substrate meso-2,6-diaminoheptanedioate, but increased activity with substrate L-alanine compared to wild-type enzyme | Erysipelothrix rhusiopathiae |
R401A | site-directed mutagenesis, almost inactive mutant | Erysipelothrix rhusiopathiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
ATP | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
0.16 | - |
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
0.21 | - |
UDP-N-acetyl-alpha-D-muramoyl-L-seryl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
0.23 | - |
UDP-N-acetyl-alpha-D-muramoyl-glycyl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
1.8 | - |
L-alanine | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
10 | - |
meso-2,6-diaminoheptanedioate | pH 9.6, 37°C, recombinant mutant MurEEr(HDNR/DNPR) | Erysipelothrix rhusiopathiae | |
64 | - |
L-alanine | pH 9.6, 37°C, recombinant mutant MurEEr(HDNR/DNPR) | Erysipelothrix rhusiopathiae | |
77 | - |
meso-2,6-diaminoheptanedioate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Erysipelothrix rhusiopathiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate | Erysipelothrix rhusiopathiae | - |
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Erysipelothrix rhusiopathiae | E7FUC9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzyme from Escherichia coli strain BL21(DE3)/pLysS by nickel affinity chromatography | Erysipelothrix rhusiopathiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetyl-alpha-D-muramoyl-glycyl-D-glutamate + L-alanine | - |
Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-glycyl-gamma-D-glutamyl-L-alanine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + (2S,6S)-2,6-diaminoheptanedioate | 7.8% activity with (2S,6S)-2,6-diaminoheptanedioate compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-(2S,6S)-2,6-diaminoheptanedioate | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + beta-chloro-L-alanine | 15% activity with beta-chloro-L-alanine compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-beta-chloro-L-alanine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + glycine | 7% activity with glycine compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-glycine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-alanine | highest activity with L-alanine compared to the other amino acids | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-L-alanine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-diaminobutyrate | 7.9% activity with L-diaminobutyrate compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-diaminobutyrate | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-homoserine | 10% activity with L-homoserine compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-homoserine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-propargylglycine | 20% activity with L-propargylglycine compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-propargylglycine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-serine | 30% activity with L-serine compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-serine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-vinylglycine | 58% activity with L-vinylglycine compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-vinylglycine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate | - |
Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate | 30% activity with meso-2,6-diaminoheptanedioate compared to L-alanine | Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-seryl-D-glutamate + L-alanine | - |
Erysipelothrix rhusiopathiae | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-seryl-gamma-D-glutamyl-L-alanine | - |
? | |
additional information | enzyme substrate specificity, overview. Poor activity with D-alanine, L-lysine, L-ornithine, L-cysteine, L-allylglycine, L-1-aminoethylphosphonic acid, L-alanine amide, L-norvaline and L-alpha-aminobutyric acid. Enzyme-ligand interaction analysis, overview | Erysipelothrix rhusiopathiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Meso-diaminopimelate-adding enzyme | - |
Erysipelothrix rhusiopathiae |
MurE | - |
Erysipelothrix rhusiopathiae |
MurEEr | - |
Erysipelothrix rhusiopathiae |
peptidoglycan MurE ligase | - |
Erysipelothrix rhusiopathiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Erysipelothrix rhusiopathiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.03 | - |
L-alanine | pH 9.6, 37°C, recombinant mutant MurEEr(HDNR/DNPR) | Erysipelothrix rhusiopathiae | |
1.83 | - |
ATP | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
2.83 | - |
L-alanine | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
3.33 | - |
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
3.67 | - |
UDP-N-acetyl-alpha-D-muramoyl-L-seryl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
4.17 | - |
meso-2,6-diaminoheptanedioate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
4.83 | - |
meso-2,6-diaminoheptanedioate | pH 9.6, 37°C, recombinant mutant MurEEr(HDNR/DNPR) | Erysipelothrix rhusiopathiae | |
8.83 | - |
UDP-N-acetyl-alpha-D-muramoyl-glycyl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.6 | - |
assay at | Erysipelothrix rhusiopathiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Erysipelothrix rhusiopathiae |
General Information | Comment | Organism |
---|---|---|
evolution | the DNPR motif that is characteristic of meso-diaminopimelate-adding enzymes is replaced by HDNR | Erysipelothrix rhusiopathiae |
additional information | the HDNR motif plays a role in the interaction of the enzyme with L-alanine and meso-diaminopimelic acid. ENzyme structure homology modeling | Erysipelothrix rhusiopathiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
L-alanine | pH 9.6, 37°C, recombinant mutant MurEEr(HDNR/DNPR) | Erysipelothrix rhusiopathiae | |
0.054 | - |
meso-2,6-diaminoheptanedioate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
0.483 | - |
meso-2,6-diaminoheptanedioate | pH 9.6, 37°C, recombinant mutant MurEEr(HDNR/DNPR) | Erysipelothrix rhusiopathiae | |
1.57 | - |
L-alanine | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
11.44 | - |
ATP | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
17.48 | - |
UDP-N-acetyl-alpha-D-muramoyl-L-seryl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
20.81 | - |
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae | |
38.39 | - |
UDP-N-acetyl-alpha-D-muramoyl-glycyl-D-glutamate | pH 9.6, 37°C, recombinant His-tagged enzyme | Erysipelothrix rhusiopathiae |