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Literature summary for 6.3.2.13 extracted from

  • Mizuno, Y.; Ito, E.
    Diaminopimelic-adding enzyme (Bacillus cereus) (1971), Methods Enzymol., 17B, 150-154.
No PubMed abstract available
Search for more literature for 6.3.2.13

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 meso-2,6-diaminoheptanedioate
-
 Bacillus cereus
0.32
-
 UDP-N-MurNAc-L-Ala-D-Glu
-
 Bacillus cereus
0.34
-
 ATP
-
 Bacillus cereus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ can partially substitute for Mg2+ Bacillus cereus
Co2+ can partially substitute for Mg2+ Bacillus cereus
K+ K+ or NH4+ required Bacillus cereus
Mg2+ Mg2+ or Mn2+ required Bacillus cereus
Mg2+ optimal concentration of Mg2+ is 10 mM Bacillus cereus
Mn2+ Mg2+ or Mn2+ required. With Mn2+ maximal activity is found at concentrations to half that of ATP Bacillus cereus
NH4+ K+ or NH4+ required Bacillus cereus
Zn2+ can partially substitute for Mg2+ Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.15
-
-
 Bacillus cereus

Storage Stability

Storage Stability Organism
-18°C, retains more than 80% of the activity after 3 months Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate r Bacillus cereus ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
 ?
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate specific for UDP-N-MurNAc-L-Ala-D-Glu and meso-2,6-diaminoheptanedioate Bacillus cereus ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
 ?
UTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate 16% of the activity relative to ATP Bacillus cereus UDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
 Bacillus cereus