Application | Comment | Organism |
---|---|---|
medicine | target for antimalarial therapy | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 cells | Plasmodium falciparum |
expression of His-tagged protein in Escherichia coli | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-[[(4-[[(2-amino-4-oxo-3,4-dihydropteridin-6-yl)methyl]amino]phenyl)(hydroxy)phosphoryl]methyl]pentanedioic acid | selective for dihydrofolate synthase activity | Plasmodium falciparum | |
2-[[[(3S)-3-[(4-[[(2-amino-4-oxo-3,4-dihydropteridin-6-yl)methyl]amino]benzoyl)amino]-3-carboxypropyl](hydroxy)phosphoryl]methyl]pentanedioic acid | - |
Plasmodium falciparum | |
additional information | not inhibitory: methotrexate | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | non-standard kinetics at high glutamate and ATP concentrations, being partially inhibited by increasing concentrations of its principal substrate, dihydropteroate. Binding of dihydropteroate to the catalytic and inhibitory sites exhibits dissociation constants of 0.50 microM and 1.25 microM, respectively. Under lower co-substrate concentrations, data fit the Michaelis-Menten equation | Plasmodium falciparum | |
0.00088 | - |
dihydropteroate | - |
Plasmodium falciparum | |
0.00088 | - |
dihydropteroate | pH 10.0, 37°C | Plasmodium falciparum | |
0.00597 | - |
glutamate | - |
Plasmodium falciparum | |
0.006 | - |
L-Glu | pH 10.0, 37°C | Plasmodium falciparum | |
0.0228 | - |
ATP | - |
Plasmodium falciparum | |
0.0228 | - |
ATP | pH 10.0, 37°C | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
1 * 62400, calculated for His-tagged recombinant protein, 1 * 62000, SDS-PAGE | Plasmodium falciparum |
62400 | - |
1 * 62400, calculated for His-tagged recombinant protein, 1 * 62000, SDS-PAGE | Plasmodium falciparum |
90000 | - |
gel filtration, His-tagged recombinant protein | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Plasmodium falciparum | - |
bifunctional dihydrofolate synthase-folylpolyglutamate synthase | - |
Purification (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 7,8-dihydropteroate + L-Glu | - |
Plasmodium falciparum | ADP + phosphate + 7,8-dihydrofolate | - |
? | |
ATP + L-glutamate + 7,8-dihydropteroate | assay at pH 10, 37°C | Plasmodium falciparum | ADP + phosphate + 7,8-dihydropteroylglutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Plasmodium falciparum |
monomer | 1 * 62400, calculated for His-tagged recombinant protein, 1 * 62000, SDS-PAGE | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
DHFS | - |
Plasmodium falciparum |
dihydrofolate synthase | - |
Plasmodium falciparum |
PfDHFS-FPGS | bifunctional protein together with folypolyglutamate synthase, EC 6.3.2.17 | Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
assay at | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00014 | - |
2-[[(4-[[(2-amino-4-oxo-3,4-dihydropteridin-6-yl)methyl]amino]phenyl)(hydroxy)phosphoryl]methyl]pentanedioic acid | - |
Plasmodium falciparum | |
0.00014 | - |
2-[[(4-[[(2-amino-4-oxo-3,4-dihydropteridin-6-yl)methyl]amino]phenyl)(hydroxy)phosphoryl]methyl]pentanedioic acid | pH 10.0, 37°C | Plasmodium falciparum | |
0.00169 | - |
2-[[[(3S)-3-[(4-[[(2-amino-4-oxo-3,4-dihydropteridin-6-yl)methyl]amino]benzoyl)amino]-3-carboxypropyl](hydroxy)phosphoryl]methyl]pentanedioic acid | - |
Plasmodium falciparum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000041 | - |
- |
Plasmodium falciparum | 2-[[(4-[[(2-amino-4-oxo-3,4-dihydropteridin-6-yl)methyl]amino]phenyl)(hydroxy)phosphoryl]methyl]pentanedioic acid |
Organism | Comment | Expression |
---|---|---|
Plasmodium falciparum | expressed as single bifunctional protein with folypolyglutamate synthase, EC 6.3.2.17 | additional information |