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Literature summary for 6.3.2.12 extracted from

  • Salcedo, E.; Cortese, J.F.; Plowe, C.V.; Sims, P.F.G.; Hyde, J.E.
    A bifunctional dihydrofolate synthetase--folylpolyglutamate synthetase in Plasmodium falciparum identified by functional complementation in yeast and bacteria (2001), Mol. Biochem. Parasitol., 112, 239-252.
    View publication on PubMed
Search for more literature for 6.3.2.12

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Saccharomyces cerevisiae Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
additional information removal of the first 16 or 40 residues of the protein does not abolish the enzyme activity Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q9U738 the gene encodes a protein with both dihydrofolate synthase and folylpolyglutamate synthase activities; 3D7, K1, Tak9, M24, K39, HB3, W282, V1/S, Dd2 and FCB lines
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 7,8-dihydropteroate + L-Glu
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 Plasmodium falciparum ADP + phosphate + 7,8-dihydrofolate
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 ?
additional information the expression of the enzyme in Escherichia coli and Saccharomyces cerevisiae defective strains restores Escherichia coli cells to methionine and glycine prototrophy on minimal medium, but cannot entirely compensate for the loss of the native homologue in Saccharomyces cerevisiae. The malarial gene encodes a protein carrying dihydrofolate synthetase and folylpolyglutamate synthetase activities, both needed for the de novo folate synthesis Plasmodium falciparum ?
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 ?

Synonyms

Synonyms Comment Organism
DHFS
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 Plasmodium falciparum
dihydrofolate synthetase-folylpolyglutamate synthetase
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 Plasmodium falciparum
H2-folate synthetase
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 Plasmodium falciparum