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Literature summary for 6.3.2.12 extracted from

  • Patel, O.; Castelli, L.; Fernley, R.; Coloe, P.; Macreadie, I.
    Production of a functional dihydrofolate synthase with a cleavable poly-His tag in Saccharomyces cerevisiae (2002), Biotechnol. Lett., 24, 657-662.
    View publication on PubMed
Search for more literature for 6.3.2.12

Application

Application Comment Organism
biotechnology production of active dihidrofolate synthase in milligram scale Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression of a histidine-tagged recombinant enzyme in a Saccharomyces cerevisiae lacking a DHFS functional gene Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 CUY13 strain
-
Saccharomyces cerevisiae CUY13
-
 CUY13 strain
-

Purification (Commentary)

Purification (Comment) Organism
purification of a histidine-tagged recombinant enzyme using metal-affinity chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 7,8-dihydropteroate + L-Glu
-
 Saccharomyces cerevisiae ADP + phosphate + 7,8-dihydrofolate
-
 ?
ATP + 7,8-dihydropteroate + L-Glu
-
 Saccharomyces cerevisiae CUY13 ADP + phosphate + 7,8-dihydrofolate
-
 ?

Synonyms

Synonyms Comment Organism
DHFS
-
 Saccharomyces cerevisiae