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Literature summary for 6.3.2.12 extracted from

  • Iwai, K.; Ikeda, M.
    Purification and properties of the dihydrofolate synthetase from pea seedlings (1975), J. Nutr. Sci. Vitaminol., 21, 7-18.
    View publication on PubMed
Search for more literature for 6.3.2.12

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 7,8-dihydropteroate
-
 Pisum sativum
0.1
-
 ATP
-
 Pisum sativum
1.5
-
 Glu
-
 Pisum sativum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ K+ is most effective Pisum sativum
K+ monovalent cation requirement is satisfied by K+, Rb+ or NH4+ Pisum sativum
Mg2+ Km: 1.1 mM Pisum sativum
Mn2+ Km: 0.063 mM Pisum sativum
Mn2+ divalent cation required Pisum sativum
NH4+
-
 Pisum sativum
Rb+ monovalent cation requirement is satisfied by K+, Rb+ or NH4+ Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 gel filtration Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.007
-
-
 Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 7,8-dihydropteroate + L-Glu
-
 Pisum sativum ADP + phosphate + 7,8-dihydrofolate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
 Pisum sativum

pH Range

pH Minimum pH Maximum Comment Organism
7.5 11 7.5: about 50% of maximal activity, 11.0: about 80% of activity maximum Pisum sativum