General Stability | Organism |
---|---|
lyophilized enzyme is very stable | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Escherichia coli | |
2,3-Diaminopropanoate | slight | Escherichia coli | |
2-hydroxybutanoate | slight | Escherichia coli | |
2-Hydroxypropanoate | slight | Escherichia coli | |
3-hydroxybutanoate | slight | Escherichia coli | |
3-hydroxypropanoate | slight | Escherichia coli | |
4-Amino-3-hydroxybutanoate | - |
Escherichia coli | |
4-aminobutanoate | - |
Escherichia coli | |
5-Aminopentanoate | slight | Escherichia coli | |
6-aminohexanoate | slight | Escherichia coli | |
Ag+ | - |
Escherichia coli | |
anserine | slight | Escherichia coli | |
Ba2+ | - |
Escherichia coli | |
Ca2+ | - |
Escherichia coli | |
carnosine | slight | Escherichia coli | |
Co2+ | - |
Escherichia coli | |
Cu2+ | - |
Escherichia coli | |
EDTA | - |
Escherichia coli | |
EGTA | - |
Escherichia coli | |
Fe2+ | - |
Escherichia coli | |
gluconate | 32% inhibition at 1 mM | Escherichia coli | |
glycolate | 30% inhibition at 1 mM | Escherichia coli | |
Hg2+ | - |
Escherichia coli | |
iodoacetate | - |
Escherichia coli | |
L-Ala | - |
Escherichia coli | |
L-cysteate | - |
Escherichia coli | |
L-Val | - |
Escherichia coli | |
Lactate | - |
Escherichia coli | |
Ni2+ | slight | Escherichia coli | |
Sn2+ | slight | Escherichia coli | |
Sr2+ | slight | Escherichia coli | |
taurine | - |
Escherichia coli | |
Zn2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
D-pantoate | - |
Escherichia coli | |
0.1 | - |
ATP | - |
Escherichia coli | |
0.15 | - |
beta-Alanine | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | combination of divalent and monovalent cation required | Escherichia coli | |
Mg2+ | divalent cation required, Mg2+ or Mn2+, Km: 2.0 mM. Optimal concentration: 10 mM, inhibition above | Escherichia coli | |
Mn2+ | combination of divalent and monovalent cation required | Escherichia coli | |
Mn2+ | divalent cation required, Mg2+ or Mn2+. Optimal concentration: 5 mM, inhibition above | Escherichia coli | |
NH4+ | combination of divalent and monovalent cation required | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate | bi uni uni bi ping pong mechanism | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, stable for 6 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | - |
Escherichia coli | AMP + diphosphate + (R)-pantothenate | - |
? | |
CTP + (R)-pantoate + beta-alanine | 14% of the activity relative to ATP | Escherichia coli | CMP + diphosphate + (R)-pantothenate | - |
? | |
GTP + (R)-pantoate + beta-alanine | 12% of the activity relative to ATP | Escherichia coli | GMP + diphosphate + (R)-pantothenate | - |
? | |
ITP + (R)-pantoate + beta-alanine | 14% of the activity relative to ATP | Escherichia coli | IMP + diphosphate + (R)-pantothenate | - |
? | |
UTP + (R)-pantoate + beta-alanine | 16% of the activity relative to ATP | Escherichia coli | UMP + diphosphate + (R)-pantothenate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, complete loss of activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | 15 min, 40°C, stable | Escherichia coli |