Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pantothenate | product inhibition | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
D-pantoate | 25°C, pH 7.8 | Mycobacterium tuberculosis | |
0.8 | - |
beta-Alanine | 25°C, pH 7.8 | Mycobacterium tuberculosis | |
2.6 | - |
ATP | 25°C, pH 7.8 | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | maximal activity at 10 mM, slight activation | Mycobacterium tuberculosis | |
Mg2+ | maximal activity at 10 mM, very strong activation | Mycobacterium tuberculosis | |
Mn2+ | maximal activity at 10 mM, very strong activation | Mycobacterium tuberculosis | |
Ni2+ | maximal activity at approx. 3 mM, slight activation | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32677 | - |
2 * 32677, deduced from nucleotide sequence | Mycobacterium tuberculosis |
33000 | - |
2 * 33000, SDS-PAGE | Mycobacterium tuberculosis |
65000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | Mycobacterium tuberculosis | last step in pantothenate biosynthesis pathway | AMP + diphosphate + (R)-pantothenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
PanC is essential for growth | - |
Purification (Comment) | Organism |
---|---|
recombinant PanC | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | - |
Mycobacterium tuberculosis | AMP + diphosphate + (R)-pantothenate | - |
? | |
ATP + (R)-pantoate + beta-alanine | last step in pantothenate biosynthesis pathway | Mycobacterium tuberculosis | AMP + diphosphate + (R)-pantothenate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33000, SDS-PAGE | Mycobacterium tuberculosis |
dimer | 2 * 32677, deduced from nucleotide sequence | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
PanC | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
beta-Alanine | 25°C, pH 7.8 | Mycobacterium tuberculosis | |
3.4 | - |
D-pantoate | 25°C, pH 7.8 | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
pantothenate | 25°C, pH 7.8, vs. beta-alanine | Mycobacterium tuberculosis | |
5.7 | - |
pantothenate | 25°C, pH 7.8, vs. D-pantoate | Mycobacterium tuberculosis | |
7.6 | - |
pantothenate | 25°C, pH 7.8, vs. ATP | Mycobacterium tuberculosis |