Cloned (Comment) | Organism |
---|---|
- |
Oryza sativa |
expression Escherichia coli | Lotus japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
(R)-pantoate | 25°C, pH 8.0 | Lotus japonicus | |
0.44 | - |
beta-Alanine | 25°C, pH 8.0, at 20 mM pantoate | Lotus japonicus | |
0.98 | - |
beta-Alanine | 25°C, pH 8.0, at 0.5 mM pantoate | Lotus japonicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Lotus japonicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33900 | - |
x * 33900, deduced from nucleotide sequence | Oryza sativa |
34000 | - |
2 * 34000, SDS-PAGE | Lotus japonicus |
72800 | - |
gel filtration | Lotus japonicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | Lotus japonicus | - |
AMP + diphosphate + (R)-pantothenate | - |
? | |
ATP + (R)-pantoate + beta-alanine | Oryza sativa | - |
AMP + diphosphate + (R)-pantothenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lotus japonicus | O24035 | - |
- |
Oryza sativa | O24210 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant pantothenate snthetase | Lotus japonicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.36 | - |
- |
Lotus japonicus |
Storage Stability | Organism |
---|---|
-20°C, no loss of activity | Lotus japonicus |
4°C, 4 weeks, 25% loss of activity | Lotus japonicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | - |
Lotus japonicus | AMP + diphosphate + (R)-pantothenate | - |
? | |
ATP + (R)-pantoate + beta-alanine | - |
Oryza sativa | AMP + diphosphate + (R)-pantothenate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33900, deduced from nucleotide sequence | Oryza sativa |
dimer | 2 * 34000, SDS-PAGE | Lotus japonicus |
Synonyms | Comment | Organism |
---|---|---|
Pantothenate synthetase | - |
Lotus japonicus |
Pantothenate synthetase | - |
Oryza sativa |
PS | - |
Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.62 | - |
(R)-pantoate | 25°C, pH 8.0 | Lotus japonicus | |
6.08 | - |
(R)-pantoate | 25°C, pH 8.0 | Lotus japonicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
in Tris-HCl | Lotus japonicus |
8 | - |
in potassium phosphate | Lotus japonicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 50% of maximal activity at pH 7.0, 15% of maximal activity at pH 6.0 and pH 9.0 respectively | Lotus japonicus |
7.1 | 9 | in Tris-HCl, no activity at pH 7.0, 75% of maximal activity at pH 9.0 | Lotus japonicus |