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Literature summary for 6.3.1.9 extracted from

  • Ariyanayagam, M.R.; Oza, S.L.; Guther, M.L.S.; Fairlamb, A.H.
    Phenotypic analysis of trypanothione synthetase knockdown in the African trypanosome (2005), Biochem. J., 391, 425-432.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination, cloning and RNAi transfection, expression in Escherichia coli Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
additional information enzyme downregulation leads to decreased growth rate of the parasite, increased H2O2 and tert-butylhydroperoxide sensitivity, enzyme knockdown leads to depletion of both trypanothione and glutathionylspermidine, and glutathione and polyamine accumulation, RNA interference method, overview Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
kinetoplast
-
Trypanosoma brucei 20023
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 glutathione + spermidine + 2 ATP Trypanosoma brucei important reaction in defense against chemical and oxidant stress, thiol redox homeostasis, ribonucleotide metabolism, and drug resistance in parasitic kinetoplastids N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
?
glutathione + glutathionylspermidine + ATP Trypanosoma brucei trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
glutathione + spermidine + ATP Trypanosoma brucei trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei glutathionylspermidine + ADP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
bifunctional enzyme with glutathionylspermidine synthase and trypanothione synthase activity
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 glutathione + spermidine + 2 ATP important reaction in defense against chemical and oxidant stress, thiol redox homeostasis, ribonucleotide metabolism, and drug resistance in parasitic kinetoplastids Trypanosoma brucei N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
?
2 glutathione + spermidine + 2 ATP trypanothione synthase overall reaction Trypanosoma brucei N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
?
glutathione + glutathionylspermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei Trypanosoma brucei N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
glutathione + spermidine + ATP trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei Trypanosoma brucei glutathionylspermidine + ADP + phosphate
-
?

Synonyms

Synonyms Comment Organism
TryS
-
Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
ATP
-
Trypanosoma brucei