Cloned (Comment) | Organism |
---|---|
DNA sequence determination, cloning and RNAi transfection, expression in Escherichia coli | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme downregulation leads to decreased growth rate of the parasite, increased H2O2 and tert-butylhydroperoxide sensitivity, enzyme knockdown leads to depletion of both trypanothione and glutathionylspermidine, and glutathione and polyamine accumulation, RNA interference method, overview | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
kinetoplast | - |
Trypanosoma brucei | 20023 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 glutathione + spermidine + 2 ATP | Trypanosoma brucei | important reaction in defense against chemical and oxidant stress, thiol redox homeostasis, ribonucleotide metabolism, and drug resistance in parasitic kinetoplastids | N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate | - |
? | |
glutathione + glutathionylspermidine + ATP | Trypanosoma brucei | trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei | N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | Trypanosoma brucei | trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei | glutathionylspermidine + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
bifunctional enzyme with glutathionylspermidine synthase and trypanothione synthase activity | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 glutathione + spermidine + 2 ATP | important reaction in defense against chemical and oxidant stress, thiol redox homeostasis, ribonucleotide metabolism, and drug resistance in parasitic kinetoplastids | Trypanosoma brucei | N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate | - |
? | |
2 glutathione + spermidine + 2 ATP | trypanothione synthase overall reaction | Trypanosoma brucei | N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate | - |
? | |
glutathione + glutathionylspermidine + ATP | trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei | Trypanosoma brucei | N1,N8-bis(glutathionyl)spermidine + ADP + phosphate | - |
? | |
glutathione + spermidine + ATP | trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei | Trypanosoma brucei | glutathionylspermidine + ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TryS | - |
Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Trypanosoma brucei |