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Literature summary for 6.3.1.8 extracted from

  • Tetaud, E.; Manai, F.; Barrett, M.P.; Nadeau, K.; Walsh, C.T.; Fairlamb, A.H.
    Cloning and characterization of the two enzymes responsible for trypanothione biosynthesis in Crithidia fasciculata (1998), J. Biol. Chem., 273, 19383-19390.
    View publication on PubMed
Search for more literature for 6.3.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Saccharomyces cerevisiae Crithidia fasciculata
overexpression in Escherichia coli and Saccharomyces cerevisiae of the gene Cf-GSS yields insoluble proteins Crithidia fasciculata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80340
-
 predicted from sequence Crithidia fasciculata
80640
-
 MALDI-TOF MS Crithidia fasciculata
90000
-
 1 * 90000, SDS-PAGE Crithidia fasciculata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
gamma-L-Glutamyl-L-cysteinyl-glycine + spermidine + ATP Crithidia fasciculata synthesis of trypanothione, which is involved in maintaining intracellular thiol redox and in defense against oxidants ?
-
 ?

Organism

Organism UniProt Comment Textmining
Crithidia fasciculata
-
-
-
Crithidia fasciculata P90518
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme cloned in Escherichia coli and Saccharomyces cerevisiae is inactive Crithidia fasciculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gamma-L-Glu-L-Cys-Gly + spermidine + ATP
-
 Crithidia fasciculata N1-(gamma-L-Glu-L-Cys-Gly)-spermidine + ADP + phosphate
-
 ?
gamma-L-Glu-L-Cys-Gly + spermidine + MgATP2-
-
 Crithidia fasciculata N1-(gamma-L-Glu-L-Cys-Gly)-spermidine + MgADP- + phosphate
-
 ?
gamma-L-Glutamyl-L-cysteinyl-glycine + spermidine + ATP synthesis of trypanothione, which is involved in maintaining intracellular thiol redox and in defense against oxidants Crithidia fasciculata ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 90000, SDS-PAGE Crithidia fasciculata