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Literature summary for 6.3.1.5 extracted from

  • Spencer, R.L.; Preiss, J.
    Biosynthesis of diphosphopyridine nucleotide. The purification and the properties of diphosphopyridine nucleotide synthetase from Escherichia coli (1967), J. Biol. Chem., 242, 385-392.
    View publication on PubMed
Search for more literature for 6.3.1.5

Inhibitors

Inhibitors Comment Organism Structure
adenosine
-
 Escherichia coli
Decoyinine
-
 Escherichia coli
Mn2+ can satisfy the metal ion requirement at low concentrations, at high concentrations Mn2+ markedly inhibits Escherichia coli
Psicofuranine i.e. 9-D-psicofuranosyl-6-aminopurine, each substrate provides some protection Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
 NH3
-
 Escherichia coli
16
-
 L-Gln
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ can satisfy the metal ion requirement, Mn2+ is more effective than Mg2+ at low concentrations, at high concentrations Mn2+ markedly inhibits, but Mg2+ does not Escherichia coli
Mn2+ can satisfy the metal ion requirement, Mn2+ is more effective than Mg2+ at low concentrations, at high concentrations Mn2+ markedly inhibits, but Mg2+ does not Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.9
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + Asp slightly active as nitrogen donor Escherichia coli AMP + diphosphate + NAD+ + oxaloacetate
-
 ?
ATP + deamido-NAD+ + Asp slightly active as nitrogen donor Escherichia coli B / ATCC 11303 AMP + diphosphate + NAD+ + oxaloacetate
-
 ?
ATP + deamido-NAD+ + Glu slightly active as nitrogen donor Escherichia coli AMP + diphosphate + NAD+ + 2-oxoglutarate
-
 ?
ATP + deamido-NAD+ + Glu slightly active as nitrogen donor Escherichia coli B / ATCC 11303 AMP + diphosphate + NAD+ + 2-oxoglutarate
-
 ?
ATP + deamido-NAD+ + L-Gln
-
 Escherichia coli AMP + diphosphate + NAD+ + Glu
-
 ?
ATP + deamido-NAD+ + L-Gln
-
 Escherichia coli B / ATCC 11303 AMP + diphosphate + NAD+ + Glu
-
 ?
ATP + deamido-NAD+ + NH3 NH3 is the preferred nitrogen donor Escherichia coli AMP + diphosphate + NAD+
-
 ?
ATP + deamido-NAD+ + NH3 NH3 is the preferred nitrogen donor Escherichia coli B / ATCC 11303 AMP + diphosphate + NAD+
-
 ?
dATP + deamido-NAD+ + NH3 20% of the activity relative to ATP Escherichia coli dAMP + diphosphate + NAD+
-
 ?
dATP + deamido-NAD+ + NH3 20% of the activity relative to ATP Escherichia coli B / ATCC 11303 dAMP + diphosphate + NAD+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 8.6
-
 Escherichia coli