Cloned (Comment) | Organism |
---|---|
functional expression in Escherichia coli strain M-15 as His-tagged enzyme, secretion to the medium | Streptococcus sobrinus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion | Streptococcus sobrinus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptococcus sobrinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, recombinant His-tagged enzyme, SDS-PAGE | Streptococcus sobrinus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + NH3 | Streptococcus sobrinus | - |
AMP + diphosphate + NAD+ | - |
? | |
ATP + deamido-NAD+ + NH3 | Streptococcus sobrinus 6715 | - |
AMP + diphosphate + NAD+ | - |
? | |
additional information | Streptococcus sobrinus | enzyme induces lymphocyte polyclonal activation, it stimulates murine B cells after in vitro treatment of spleen cell cultures | ? | - |
? | |
additional information | Streptococcus sobrinus 6715 | enzyme induces lymphocyte polyclonal activation, it stimulates murine B cells after in vitro treatment of spleen cell cultures | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus sobrinus | - |
- |
- |
Streptococcus sobrinus 6715 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant as His-tagged enzyme from Escherichia coli | Streptococcus sobrinus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.49 | - |
purified recombinant His-tagged enzyme | Streptococcus sobrinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + NH3 | - |
Streptococcus sobrinus | AMP + diphosphate + NAD+ | - |
? | |
ATP + deamido-NAD+ + NH3 | - |
Streptococcus sobrinus 6715 | AMP + diphosphate + NAD+ | - |
? | |
additional information | enzyme induces lymphocyte polyclonal activation, it stimulates murine B cells after in vitro treatment of spleen cell cultures | Streptococcus sobrinus | ? | - |
? | |
additional information | enzyme induces lymphocyte polyclonal activation, it stimulates murine B cells after in vitro treatment of spleen cell cultures | Streptococcus sobrinus 6715 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, recombinant His-tagged enzyme, SDS-PAGE | Streptococcus sobrinus |
Synonyms | Comment | Organism |
---|---|---|
NAD synthase | - |
Streptococcus sobrinus |
NAD synthetase | - |
Streptococcus sobrinus |
Nicotinamide adenine dinucleotide synthetase | - |
Streptococcus sobrinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Streptococcus sobrinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
10 min, inactivation | Streptococcus sobrinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Streptococcus sobrinus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | required | Streptococcus sobrinus |