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Literature summary for 6.3.1.4 extracted from
- Asparagine synthetase from gamma-irradiated potatoes (1969), Arch. Biochem. Biophys., 133, 208-215.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Asn | - |
Solanum tuberosum |  |
| CuSO4 | - |
Solanum tuberosum | |
| EDTA | - |
Solanum tuberosum | |
| hydroxylamine | above 400 mM | Solanum tuberosum | |
| MoO42- | - |
Solanum tuberosum | |
| Na2HAsO4 | - |
Solanum tuberosum | |
| NaF | - |
Solanum tuberosum | |
| p-chloromercuribenzoate | - |
Solanum tuberosum | |
| ZnSO4 | - |
Solanum tuberosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
ATP | - |
Solanum tuberosum | |
| 26 | - |
L-Asp | - |
Solanum tuberosum | |
| 40 | - |
NH4+ | - |
Solanum tuberosum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Solanum tuberosum | |
| Mg2+ | Km: 4 mM | Solanum tuberosum | |
| Mn2+ | can replace Mg2+ | Solanum tuberosum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Solanum tuberosum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0583 | - |
- |
Solanum tuberosum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 1 week | Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Asp + hydroxylamine | maximal activity at 400 mM NH2OH | Solanum tuberosum | ADP + phosphate + beta-aspartylhydroxamate | - |
? | |
| ATP + L-Asp + NH4+ | - |
Solanum tuberosum | ADP + phosphate + L-Asn | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
Mn2+-stimulated reaction | Solanum tuberosum |
| 7.2 | - |
Mg2+-stimulated reaction | Solanum tuberosum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 8 | 5.2: about 70% of maximal Mn2+-stimulated activity, 8.0: about 35% of maximal Mn2+-stimulated activity | Solanum tuberosum |
| 6 | 8.4 | 6.0: about 70% of maximal Mg2+-stimulated activity, 8.4: about 60% of maximal Mg2+-stimulated activity | Solanum tuberosum |
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Release 2023.1 (January 2023)
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