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Literature summary for 6.3.1.4 extracted from

  • Nair, P.M.
    Asparagine synthetase from gamma-irradiated potatoes (1969), Arch. Biochem. Biophys., 133, 208-215.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Asn
-
Solanum tuberosum
CuSO4
-
Solanum tuberosum
EDTA
-
Solanum tuberosum
hydroxylamine above 400 mM Solanum tuberosum
MoO42-
-
Solanum tuberosum
Na2HAsO4
-
Solanum tuberosum
NaF
-
Solanum tuberosum
p-chloromercuribenzoate
-
Solanum tuberosum
ZnSO4
-
Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10
-
ATP
-
Solanum tuberosum
26
-
L-Asp
-
Solanum tuberosum
40
-
NH4+
-
Solanum tuberosum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Solanum tuberosum
Mn2+ can replace Mg2+ Solanum tuberosum
Mg2+ Km: 4 mM Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Solanum tuberosum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0583
-
-
Solanum tuberosum

Storage Stability

Storage Stability Organism
-20°C, stable for 1 week Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Asp + NH4+
-
Solanum tuberosum ADP + phosphate + L-Asn
-
?
ATP + L-Asp + hydroxylamine maximal activity at 400 mM NH2OH Solanum tuberosum ADP + phosphate + beta-aspartylhydroxamate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
Mg2+-stimulated reaction Solanum tuberosum
6
-
Mn2+-stimulated reaction Solanum tuberosum

pH Range

pH Minimum pH Maximum Comment Organism
5.2 8 5.2: about 70% of maximal Mn2+-stimulated activity, 8.0: about 35% of maximal Mn2+-stimulated activity Solanum tuberosum
6 8.4 6.0: about 70% of maximal Mg2+-stimulated activity, 8.4: about 60% of maximal Mg2+-stimulated activity Solanum tuberosum