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Literature summary for 6.3.1.20 extracted from
- Scavenging of cytosolic octanoic acid by mutant LplA lipoate ligases allows growth of Escherichia coli strains lacking the LipB octanoyltransferase of lipoic acid synthesis (2009), J. Bacteriol., 191, 6796-6803.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in Escherichia coli | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E116A/E312K/L328F | mutations allow a LipB knockout strain to grow on a glucose minimal medium | Escherichia coli |
| F15S/T101A/S114I | mutations allow a LipB knockout strain to grow on a glucose minimal medium | Escherichia coli |
| F35L/V113I | mutations allow a LipB knockout strain to grow on a glucose minimal medium | Escherichia coli |
| R58L/H79N | mutations allow a LipB knockout strain to grow on a glucose minimal medium | Escherichia coli |
| S221P | mutant with reduced affinity to octanoic acid | Escherichia coli |
| S221P | strain FH27, referred as lplA11, mutation allows a LipB knockout strain to grow on a glucose minimal medium | Escherichia coli |
| S8T/N63K/F78Y/A110T | mutations allow a LipB knockout strain to grow on a glucose minimal medium | Escherichia coli |
| V19L | mutant with reduced affinity to octanoic acid | Escherichia coli |
| V19L | strain FH26, referred as lplA10, mutation allows a LipB knockout strain to grow on a glucose minimal medium | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
octanoic acid | mutant S221P | Escherichia coli |  |
| 0.0104 | - |
octanoic acid | mutant V19L | Escherichia coli | |
| 0.2143 | - |
octanoic acid | wild type enzyme | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lipoic acid + ATP + apoprotein | Escherichia coli | lipoic acid is (R)-5-(1,2-dithiolan-3-yl)pentanoic acid, also called 6,8-dithiooctanoic acid or thioctic acid | diphosphate + AMP + N6-(lipoyl)-lysine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
strains MG1655 and W3110 | - |
| Escherichia coli | - |
wild type and LipB knockout strains with spontaneous mutations in lplA gene | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
| Ni-NTA column chromatography, ammonium sulfate precipitation gel filtration | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Vmax of mutant S221P is 0.0025 nmol/min | Escherichia coli |
| additional information | - |
Vmax of mutant V19L is 0.016 nmol/min | Escherichia coli |
| additional information | - |
Vmax of wild type enzyme is 0.024 nmol/min | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + octanoic acid | - |
Escherichia coli | diphosphate + octanoyl-AMP | - |
? | |
| lipoic acid + ATP + apoprotein | lipoic acid is (R)-5-(1,2-dithiolan-3-yl)pentanoic acid, also called 6,8-dithiooctanoic acid or thioctic acid | Escherichia coli | diphosphate + AMP + N6-(lipoyl)-lysine | - |
? | |
| octanoic acid + ATP | - |
Escherichia coli | diphosphate + octanoyl-AMP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipoate ligase | - |
Escherichia coli |
| lipoate-protein ligase | - |
Escherichia coli |
| LPLA | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0042 | - |
octanoic acid | mutant S221P | Escherichia coli | |
| 0.0266 | - |
octanoic acid | mutant V19L | Escherichia coli | |
| 0.04 | - |
octanoic acid | wild type enzyme | Escherichia coli | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | The LipB octanoyltransferase catalyzes the first step of lipoic acid synthesis in Escherichia coli, transfer of the octanoyl moiety from octanoyl-acyl carrier protein to the lipoyl domains of the E2 subunits of the 2-oxoacid dehydrogenases of aerobic metabolism. | Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.186 | - |
octanoic acid | wild type enzyme | Escherichia coli | |
| 1.05 | - |
octanoic acid | mutant S221P | Escherichia coli | |
| 2.55 | - |
octanoic acid | mutant V19L | Escherichia coli | |
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