Cloned (Comment) | Organism |
---|---|
from cDNA library in pTrcHis2A for expression and functional complementation in Escherichia coli strain TM137 (lplA/lipB null mutant of strain JK1) | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lipoic acid + ATP + apoprotein | Oryza sativa | two-step reaction of protein lipoylation at lysine residues with lipoyl-AMP intermediate | diphosphate + AMP + N6-(lipoyl)-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
Japonica type | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | developing, moderate expression, Northern blot | Oryza sativa | - |
leaf | northern blot | Oryza sativa | - |
additional information | transcript levels not influenced by reduced temperature (4°C), high salt (100 mM NaCl) or 72 h treatment with 100 micromolar kinetin, 100 micromolar gibberellic acid or 50 micromolar alpha-naphthalene acetic acid | Oryza sativa | - |
root | less abundant, Northern blot | Oryza sativa | - |
seed | developing seed, increasing level at 3 to 5 days after pollination followed by decreasing for 15 days, Northern blot | Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lipoic acid + ATP + apoprotein | two-step reaction of protein lipoylation at lysine residues with lipoyl-AMP intermediate | Oryza sativa | diphosphate + AMP + N6-(lipoyl)-lysine | - |
? | |
lipoic acid + ATP + apoprotein | complementation assay in LplA-deficient Escherichia coli strain TM137 (2 days, 37°C) using exogenous lipoate | Oryza sativa | diphosphate + AMP + N6-(lipoyl)-lysine | detection of lipoylated proteins in Western blot | ? |
Synonyms | Comment | Organism |
---|---|---|
lipoate-protein ligase A | - |
Oryza sativa |
lipoyl-protein ligase A | - |
Oryza sativa |
Oryza s. lipoate-protein ligase A | - |
Oryza sativa |
OsLPLA | on chromosome 8 of Oryza sativa | Oryza sativa |