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Literature summary for 6.3.1.2 extracted from

  • Glevarec, G.; Bouton, S.; Jaspard, E.; Riou, M.T.; Cliquet, J.B.; Suzuki, A.; Limami, A.M.
    Respective roles of the glutamine synthetase/glutamate synthase cycle and glutamate dehydrogenase in ammonium and amino acid metabolism during germination and post-germinative growth in the model legume Medicago truncatula (2004), Planta, 219, 286-297.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-Glu + NH4+ Medicago truncatula isoenzyme GS1b (EC 6.3.1.2) in concert with NADH-dependent GOGAT (EC 1.4.1.14) constitute the major route of assimilation of ammonium derived from reserve mobilization and glutamic acid/glutamine synthesis in germinating Medicago truncatula seeds. However, during post-germinative growth, although germination is held in darkness, expression of GS2 and Fd-GOGAT (EC 1.4.7.1) increases and expression of GS1b decreases in cotyledons but not in the embryo axis ADP + phosphate + L-Gln
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Organism

Organism UniProt Comment Textmining
Medicago truncatula
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Source Tissue

Source Tissue Comment Organism Textmining
cotyledon during post-germinative growth, expression of GS2 increases and expression of GS1b decreases in cotyledons but not in the embryo axis Medicago truncatula
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embryo during post-germinative growth, expression of GS2 increases and expression of GS1b decreases in cotyledons but not in the embryo axis Medicago truncatula
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seed isoenzyme GS1b (EC 6.3.1.2) in concert with NADH-dependent GOGAT (EC 1.4.1.14) constitute the major route of assimilation of ammonium derived from reserve mobilization and glutamic acid/glutamine synthesis in germinating Medicago truncatula seeds Medicago truncatula
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+ isoenzyme GS1b (EC 6.3.1.2) in concert with NADH-dependent GOGAT (EC 1.4.1.14) constitute the major route of assimilation of ammonium derived from reserve mobilization and glutamic acid/glutamine synthesis in germinating Medicago truncatula seeds. However, during post-germinative growth, although germination is held in darkness, expression of GS2 and Fd-GOGAT (EC 1.4.7.1) increases and expression of GS1b decreases in cotyledons but not in the embryo axis Medicago truncatula ADP + phosphate + L-Gln
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