Cloned (Comment) | Organism |
---|---|
glutamine synthetase type III, expression in Escherichia coli BL21 | Ruminococcus albus |
Protein Variants | Comment | Organism |
---|---|---|
E152A | mutant enzyme shows decreased enzymatic activity | Ruminococcus albus |
E192A | mutant enzyme shows decreased enzymatic activity | Ruminococcus albus |
E194A | mutant enzyme shows decreased enzymatic activity | Ruminococcus albus |
E308A | mutation results in an increase in activity in the forward assay compared to wild-type enzyme | Ruminococcus albus |
E380A | mutant enzyme shows decreased enzymatic activity | Ruminococcus albus |
E77A | no difference to wild-type enzyme in activity | Ruminococcus albus |
K318A | mutant enzyme shows decreased enzymatic activity | Ruminococcus albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
ADP | pH 6.4, 37°C, wild-type enzyme | Ruminococcus albus | |
1.5 | - |
hydroxylamine | pH 6.4, 37°C, mutant enzyme E380A | Ruminococcus albus | |
4.5 | - |
ATP | pH 6.4, 37°C, wild-type enzyme | Ruminococcus albus | |
4.7 | - |
hydroxylamine | pH 6.4, 37°C, wild-type enzyme | Ruminococcus albus | |
6.45 | - |
ATP | pH 6.4, 37°C, mutant enzyme E380A | Ruminococcus albus | |
6.8 | - |
L-glutamine | pH 6.4, 37°C, wild-type enzyme | Ruminococcus albus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can substitute for Mg2+ in biosynthesis of glutamine with 30% of the efficiency obtained with Mg2+ | Ruminococcus albus | |
Mg2+ | clear preference for Mg2+ in glutamine biosynthesis, optimal concentration is 50 mM | Ruminococcus albus | |
Mn2+ | optimal concentration is 1 mM, activates gamma-glutamyltransferase activity, glutamine synthetase type III. Can substitute for Mg2+ in biosynthesis of glutamine with 30% of the efficiency obtained with Mg2+ | Ruminococcus albus | |
additional information | little or no detectable activity with Mg2+, Ca2+, Co2+ or Fe2+ | Ruminococcus albus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruminococcus albus | Q4ZH57 | 8 | - |
Ruminococcus albus 8 | Q4ZH57 | 8 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme glutamine synthetase type III | Ruminococcus albus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Ruminococcus albus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH4+ | biosynthesis of glutamine | Ruminococcus albus | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH4+ | biosynthesis of glutamine | Ruminococcus albus 8 | ADP + phosphate + L-glutamine | - |
? | |
L-glutamine + hydroxylamine + ADP | gamma-glutamyltransferase activity | Ruminococcus albus | gamma-glutamylhydroxamate + NH4+ + ? | - |
? | |
L-glutamine + hydroxylamine + ADP | gamma-glutamyltransferase activity | Ruminococcus albus 8 | gamma-glutamylhydroxamate + NH4+ + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | the enzyme is present in solution as monomer and as oligomer | Ruminococcus albus |
oligomer | the enzyme is present in solution as monomer and as oligomer | Ruminococcus albus |
Synonyms | Comment | Organism |
---|---|---|
glutamine synthetase type III | - |
Ruminococcus albus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 38 | gamma-glutamyltransferase activity, glutamine synthetase type III | Ruminococcus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
glutamine synthesis | Ruminococcus albus |
6.4 | - |
gamma-glutamyltransferase activity, glutamine synthetase type III | Ruminococcus albus |