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Literature summary for 6.3.1.2 extracted from

  • Amaya, K.R.; Kocherginskaya, S.A.; Mackie, R.I.; Cann, I.K.
    Biochemical and mutational analysis of glutamine synthetase type III from the rumen anaerobe Ruminococcus albus 8 (2005), J. Bacteriol., 187, 7481-7491.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.1.2

Cloned(Commentary)

Cloned (Comment) Organism
glutamine synthetase type III, expression in Escherichia coli BL21 Ruminococcus albus

Protein Variants

Protein Variants Comment Organism
E152A mutant enzyme shows decreased enzymatic activity Ruminococcus albus
E192A mutant enzyme shows decreased enzymatic activity Ruminococcus albus
E194A mutant enzyme shows decreased enzymatic activity Ruminococcus albus
E308A mutation results in an increase in activity in the forward assay compared to wild-type enzyme Ruminococcus albus
E380A mutant enzyme shows decreased enzymatic activity Ruminococcus albus
E77A no difference to wild-type enzyme in activity Ruminococcus albus
K318A mutant enzyme shows decreased enzymatic activity Ruminococcus albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 ADP pH 6.4, 37°C, wild-type enzyme Ruminococcus albus
1.5
-
 hydroxylamine pH 6.4, 37°C, mutant enzyme E380A Ruminococcus albus
4.5
-
 ATP pH 6.4, 37°C, wild-type enzyme Ruminococcus albus
4.7
-
 hydroxylamine pH 6.4, 37°C, wild-type enzyme Ruminococcus albus
6.45
-
 ATP pH 6.4, 37°C, mutant enzyme E380A Ruminococcus albus
6.8
-
 L-glutamine pH 6.4, 37°C, wild-type enzyme Ruminococcus albus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can substitute for Mg2+ in biosynthesis of glutamine with 30% of the efficiency obtained with Mg2+ Ruminococcus albus
Mg2+ clear preference for Mg2+ in glutamine biosynthesis, optimal concentration is 50 mM Ruminococcus albus
Mn2+ optimal concentration is 1 mM, activates gamma-glutamyltransferase activity, glutamine synthetase type III. Can substitute for Mg2+ in biosynthesis of glutamine with 30% of the efficiency obtained with Mg2+ Ruminococcus albus
additional information little or no detectable activity with Mg2+, Ca2+, Co2+ or Fe2+ Ruminococcus albus

Organism

Organism UniProt Comment Textmining
Ruminococcus albus Q4ZH57 8
-
Ruminococcus albus 8 Q4ZH57 8
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme glutamine synthetase type III Ruminococcus albus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Ruminococcus albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + NH4+ biosynthesis of glutamine Ruminococcus albus ADP + phosphate + L-glutamine
-
 ?
ATP + L-glutamate + NH4+ biosynthesis of glutamine Ruminococcus albus 8 ADP + phosphate + L-glutamine
-
 ?
L-glutamine + hydroxylamine + ADP gamma-glutamyltransferase activity Ruminococcus albus gamma-glutamylhydroxamate + NH4+ + ?
-
 ?
L-glutamine + hydroxylamine + ADP gamma-glutamyltransferase activity Ruminococcus albus 8 gamma-glutamylhydroxamate + NH4+ + ?
-
 ?

Subunits

Subunits Comment Organism
monomer the enzyme is present in solution as monomer and as oligomer Ruminococcus albus
oligomer the enzyme is present in solution as monomer and as oligomer Ruminococcus albus

Synonyms

Synonyms Comment Organism
glutamine synthetase type III
-
 Ruminococcus albus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 38 gamma-glutamyltransferase activity, glutamine synthetase type III Ruminococcus albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
 glutamine synthesis Ruminococcus albus
6.4
-
 gamma-glutamyltransferase activity, glutamine synthetase type III Ruminococcus albus