Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Methanocaldococcus jannaschii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | about 50% of maximal activity at 40°C and at 70°C | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | - |
midpoint temperature of pure enzyme, none of the small molecules tested significantly stabilizes the enzyme. Stabilization by Escherichia coli GroEL or ribosomal protein L2. Protein-protein interactions appear to be the dominant factor in stabilizing the archael enzyme at the growth temperature (85°C) | Methanocaldococcus jannaschii |