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Literature summary for 6.3.1.2 extracted from

  • Bielawski, W.
    Purification and partial characterization of glutamine synthetase isoforms from Triticale seedlings (1994), Acta Biochim. Pol., 41, 397-404.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast chloroplastic isoform GS2 Secale cereale x Triticum aestivum 9507
-
cytoplasm cytoplasmic isoform GS1 Secale cereale x Triticum aestivum 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+ in activation Secale cereale x Triticum aestivum
Mg2+ divalent metal ion required, Mg2+ is most effective. Maximal activation of GS1 at 5 mM and of GS2 at 20 mM Secale cereale x Triticum aestivum
Mn2+ can partially replace Mg2+ in activation Secale cereale x Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
8 * 38000, isoform GS1, SDS-PAGE Secale cereale x Triticum aestivum
48000
-
8 * 48000, isoform GS2, SDS-PAGE Secale cereale x Triticum aestivum
305000
-
isoform GS1, gel filtration Secale cereale x Triticum aestivum
385200
-
isoform GS2, gel filtration Secale cereale x Triticum aestivum

Organism

Organism UniProt Comment Textmining
Secale cereale x Triticum aestivum
-
variety Malno
-

Purification (Commentary)

Purification (Comment) Organism
cytoplasmic isoform GS1 and chloroplastic isoform GS2 Secale cereale x Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
shoot of seedlings Secale cereale x Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.5
-
chloroplastic isoform GS2 Secale cereale x Triticum aestivum
35.1
-
cytoplasmic isoform GS1 Secale cereale x Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
Secale cereale x Triticum aestivum ADP + phosphate + L-Gln
-
?

Subunits

Subunits Comment Organism
octamer 8 * 48000, isoform GS2, SDS-PAGE Secale cereale x Triticum aestivum
octamer 8 * 38000, isoform GS1, SDS-PAGE Secale cereale x Triticum aestivum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
isoform GS1, Mn2+-activated enzyme Secale cereale x Triticum aestivum
5.4
-
isoform GS2, Mn2+-activated enzyme Secale cereale x Triticum aestivum
6.8
-
isoform GS1, Co2+-activated enzyme Secale cereale x Triticum aestivum
7
-
isoform GS2, Co2+-activated enzyme Secale cereale x Triticum aestivum
7.7
-
isoform GS1, Mg2+-activated enzyme Secale cereale x Triticum aestivum
8.2
-
isoform GS2, Mg2+-activated enzyme Secale cereale x Triticum aestivum