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Literature summary for 6.3.1.2 extracted from
- Some properties of glutamate dehydrogenase, glutamine synthetase and glutamate synthase from Corynebacterium callunae (1992), Arch. Microbiol., 158, 35-41.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ala | - |
Corynebacterium callunae |  |
| AMP | no inhibition | Corynebacterium callunae | |
| ATP | - |
Corynebacterium callunae | |
| Co2+ | 0.5-60 mM, inhibition of Mn2+-dependent transferase activity | Corynebacterium callunae | |
| Gly | - |
Corynebacterium callunae | |
| Mg2+ | between 0.5-60 mM, inhibition of Mn2+-dependent transferase activity | Corynebacterium callunae | |
| Ni2+ | 0.5-60 mM, inhibition of Mn2+-dependent transferase activity | Corynebacterium callunae | |
| Ser | - |
Corynebacterium callunae | |
| Zn2+ | 0.5-60 mM, inhibition of Mn2+-dependent transferase activity | Corynebacterium callunae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0035 | - |
ADP | - |
Corynebacterium callunae | |
| 1.46 | - |
hydroxylamine | - |
Corynebacterium callunae | |
| 15.2 | - |
Gln | - |
Corynebacterium callunae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | strongly stimulates transferase activity | Corynebacterium callunae | |
| Mg2+ | divalent cation required, specificity for Mg2+ in the biosynthetic assay | Corynebacterium callunae | |
| Mg2+ | maximal activity at 30 mM | Corynebacterium callunae | |
| Mn2+ | maximal activity at 1 mM | Corynebacterium callunae | |
| Mn2+ | required for transferase activity | Corynebacterium callunae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium callunae | - |
- |
- |
| Corynebacterium callunae NCIB 10338 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + NH4+ | - |
Corynebacterium callunae | ADP + phosphate + L-Gln | - |
? | |
| ATP + L-Glu + NH4+ | - |
Corynebacterium callunae NCIB 10338 | ADP + phosphate + L-Gln | - |
? | |
| Gln + hydroxylamine + ADP | maximal transferase activity with ADP, and good activity with AMP and GDP | Corynebacterium callunae | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? | |
| Gln + hydroxylamine + ADP | maximal transferase activity with ADP, and good activity with AMP and GDP | Corynebacterium callunae NCIB 10338 | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
biosynthetic activity | Corynebacterium callunae |
| 55 | - |
transferase activity | Corynebacterium callunae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
20 min, 20% loss of activity | Corynebacterium callunae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
biosynthetic assay | Corynebacterium callunae |
| 8 | - |
transferase activity | Corynebacterium callunae |
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