Cloned (Comment) | Organism |
---|---|
glnN gene overexpressed in Escherichia coli | Synechocystis sp. |
General Stability | Organism |
---|---|
strongly stabilized in presence of Mn2+ but not with other divalent cations. Maximal stabilization at 1 mM | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | strong, enzyme form GSIII | Synechocystis sp. | |
L-methionine sulfoximine | enzyme form GS III | Synechocystis sp. | |
Phosphinothricin | - |
Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
NH4+ | enzyme form GSIII | Synechocystis sp. | |
0.43 | - |
ATP | enzyme form GSIII | Synechocystis sp. | |
0.9 | - |
Glu | enzyme form GSIII | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
12 * 52000, enzyme form GSI, SDS-PAGE | Synechocystis sp. |
80000 | - |
6 * 80000, enzyme form GSIII, SDS-PAGE | Synechocystis sp. |
500000 | - |
enzyme form GSIII, gel filtration | Synechocystis sp. |
624000 | - |
enzyme form GSI, gel filtration | Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
strain PCC 6803 | - |
Synechocystis sp. | - |
enzyme form GSI and GSIII encoded by the gene glnA and glnB | - |
Purification (Comment) | Organism |
---|---|
enzyme form GSIII | Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.8 | - |
- |
Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | - |
Synechocystis sp. | ADP + phosphate + L-Gln | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 52000, enzyme form GSI, SDS-PAGE | Synechocystis sp. |
hexamer | 6 * 80000, enzyme form GSIII, SDS-PAGE | Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
enzyme form GSI, biosynthetic assay | Synechocystis sp. |
42 | - |
enzyme form GSIII, biosynthetic assay | Synechocystis sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
20 min, complete inactivation | Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
enzyme form GSI | Synechocystis sp. |
8.3 | - |
enzyme form GSIII, biosynthetic assay | Synechocystis sp. |