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Literature summary for 6.3.1.2 extracted from
- Characterization and glutamine synthetase isoforms from Chlorella (1985), Plant Physiol., 77, 791-794.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | 0.05 | NH4+ | isoforms GSI and GSII | Chlorella sorokiniana |  |
| 0.3 | - |
ATP | isoform GSI | Chlorella sorokiniana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
8 * 43000, isoform GSII, SDS-PAGE | Chlorella sorokiniana |
| 47000 | - |
8 * 47000, isoform GSI, SDS-PAGE | Chlorella sorokiniana |
| 360000 | - |
isoform GSII, disc gel electrophoresis | Chlorella sorokiniana |
| 398000 | - |
isoform GSI, disc gel electrophoresis | Chlorella sorokiniana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlorella sorokiniana | - |
oxygen-resistant mutant strain, isoforms GSI and GSII | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoform GSI and GSII | Chlorella sorokiniana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Chlorella sorokiniana |
| 18.5 | - |
- |
Chlorella sorokiniana |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 10% loss of activity of isoform GSII after 24 h, no loss of activity of isoform GSII | Chlorella sorokiniana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + NH4+ | - |
Chlorella sorokiniana | ADP + phosphate + L-Gln | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 43000, isoform GSII, SDS-PAGE | Chlorella sorokiniana |
| octamer | 8 * 47000, isoform GSI, SDS-PAGE | Chlorella sorokiniana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
isoform GSI and GSII are stable for 1 h | Chlorella sorokiniana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 8 | isoforms GSI and GSII | Chlorella sorokiniana |
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Release 2023.1 (January 2023)
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