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Literature summary for 6.3.1.2 extracted from

  • Caballero, F.J.; Cejudo, F.J.; Florencio, F.J.; Cardenas, J.; Castillo, F.
    Molecular and regulatory properties of glutamine synthetase from the phototrophic bacterium Rhodopseudomonas capsulata E1F1 (1985), J. Bacteriol., 162, 804-809.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.1.2

Inhibitors

Inhibitors Comment Organism Structure
Ala strong inhibition of transferase activity Rhodobacter capsulatus
Asp inhibition of biosynthetic and transferase activity Rhodobacter capsulatus
Gly inhibition of biosynthetic and transferase activity Rhodobacter capsulatus
Ser inhibition of biosynthetic and transferase activity Rhodobacter capsulatus
Val inhibition of biosynthetic and transferase activity Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 ADP transferase activity Rhodobacter capsulatus
6
-
 hydroxylamine transferase activity Rhodobacter capsulatus
12
-
 L-Gln transferase activity Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 12 * 55000, the enzyme exhibits different aggregation states with detectable oligomers of 1, 2, 3, 4, 6, 8, 10, and 12 subunits, SDS-PAGE Rhodobacter capsulatus
660000 670000 disc gel electrophoresis, pore gradient electrophoresis, sucrose density gradient centrifugation Rhodobacter capsulatus

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-
Rhodobacter capsulatus E1F1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodobacter capsulatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
153
-
 gamma-glutamyl transferase assay Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
 Rhodobacter capsulatus ADP + phosphate + L-Gln
-
 ?
ATP + L-Glu + NH4+
-
 Rhodobacter capsulatus E1F1 ADP + phosphate + L-Gln
-
 ?
Gln + hydroxylamine + ADP
-
 Rhodobacter capsulatus gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
Gln + hydroxylamine + ADP
-
 Rhodobacter capsulatus E1F1 gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?

Subunits

Subunits Comment Organism
dodecamer 12 * 55000, the enzyme exhibits different aggregation states with detectable oligomers of 1, 2, 3, 4, 6, 8, 10, and 12 subunits, SDS-PAGE Rhodobacter capsulatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information turnover number 4200, biosynthetic activity. 102000, transferase activity Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 adenylylated form, n:9, transferase activity Rhodobacter capsulatus
7
-
 intermediate form, n:6, transferase activity Rhodobacter capsulatus
7.5
-
 deadenylylated form, transferase activity Rhodobacter capsulatus